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分子别名（Synonyms）
Integrin alpha M beta 2, ITGAM&ITGB2
表达区间及表达系统（Source）
Human ITGAM&ITGB2 Heterodimer Protein, His Tag&Tag Free (IT2-H52W4) is expressed from CHO cells. It contains AA Phe 17 - Asn 1105 (ITGAM) & Gln 23 - Asn 700 (ITGB2) (Accession # P11215-2 (ITGAM) & P05107-1 (ITGB2) (H354Q)).
Predicted N-terminus: Phe 17 (ITGAM) & Gln 23 (ITGB2)
Request for sequence蛋白结构（Molecular Characterization）
Human ITGAM&ITGB2 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGAM and ITGB2, has a calculated MW of 127.2 kDa (ITGAM) and 80.2 kDa (ITGB2). Subunit ITGAM is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB2 contains no tag but a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 150-180 kDa (ITGAM) and 90-100 kDa (ITGB2) when calibrated against Star Ribbon Pre-stained Protein Marker respectively due to glycosylation.
内毒素（Endotoxin）
Less than 0.01 EU per μg by the LAL method / rFC method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
运输和存储（Shipping and Storage）
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
Human ITGAM&ITGB2 Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human ITGAM&ITGB2 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT2-H52W4) is more than 85% and the molecular weight of this protein is around 220-280 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-SPR
Human ITGAM&ITGB2 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT2-H52W4) immobilized on CM5 Chip can bind Human CFH Protein, His Tag (Cat. No. CFH-H52H3) with an affinity constant of 4.24 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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