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分子别名（Synonym）
IL-23 alpha & IL-12 beta
表达区间及表达系统（Source）
Rat IL-23 alpha& IL-12 beta Heterodimer Protein, His Tag&Tag Free (ILB-R52W3) is expressed from human 293 cells (HEK293). It contains AA Val 22 - Ala 196 & Met 23 - Ser 335 (Accession # Q91Z84 & Q9R278).
Predicted N-terminus: Val 22 & Met 23
Request for sequence蛋白结构（Molecular Characterization）
Rat IL-23 alpha& IL-12 beta Heterodimer Protein, His Tag&Tag Free, produced by co-expression of IL23A and IL12B, has a calculated MW of 21.5 kDa (IL23A) and 35.9 kDa (IL12B). Subunit IL-23A is fused with a polyhistidine tag at the C-terminus and subunit IL-12B contains no tag. The protein migrates as 23-25 kDa and 43-45 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a heterodimer.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method / rFC method.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
Rat IL-23 alpha& IL-12 beta Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Rat IL-23 alpha& IL-12 beta Heterodimer Protein, His Tag&Tag Free (Cat. No. ILB-R52W3) is more than 85% and the molecular weight of this protein is around 65-85 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Biotinylated Human IL-23 R, Fc,Avitag (Cat. No. ILR-H82F3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Rat IL-23 alpha& IL-12 beta Heterodimer Protein, His Tag&Tag Free (Cat. No. ILB-R52W3) with a linear range of 1-39 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Human IL-12 R beta 1, Fc,Avitag (Cat. No. ILB-H82F7) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Rat IL-23 alpha& IL-12 beta Heterodimer Protein, His Tag&Tag Free (Cat. No. ILB-R52W3) with a linear range of 1-39 ng/mL (QC tested).
Protocol
活性（Bioactivity）-SPR
Biotinylated Human IL-23 R, Fc,Avitag (Cat. No. ILR-H82F3) captured on Biotin CAP-Series S Sensor Chip can bind Rat IL-23 alpha& IL-12 beta Heterodimer Protein, His Tag&Tag Free (Cat. No. ILB-R52W3) with an affinity constant of 0.503 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Biotinylated Human IL-12 R beta 1, His,Avitag (Cat. No. ILB-H52E1) captured on Biotin CAP-Series S Sensor Chip can bind Rat IL-23 alpha& IL-12 beta Heterodimer Protein, His Tag&Tag Free (Cat. No. ILB-R52W3) with an affinity constant of 0.971 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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