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分子别名（Synonym）
IL-23 alpha & IL-12 beta
表达区间及表达系统（Source）
Mouse IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free (ILB-M52W7) is expressed from human 293 cells (HEK293). It contains AA Val 22 - Ala 196 (IL23A) & Met 23 - Ser 335 (IL12B) (Accession # Q9EQ14-1 (IL23A) & P43432-1 (IL12B)).
Predicted N-terminus: His (IL23A) & Met 23 (IL12B)
Request for sequence蛋白结构（Molecular Characterization）
Mouse IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free, produced by co-expression of IL-23A and IL-12B, has a calculated MW of 20.5 kDa (IL-23A) and 35.8 kDa (IL-12B). Subunit IL-23A is fused with a polyhistidine tag at the N-terminus and subunit IL-12B contains no tag. The protein migrates as 20 kDa (IL23A) & 45 kDa (IL12B) when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a heterodimer.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method / rFC method.
纯度（Purity）
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
Mouse IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Mouse IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free (Cat. No. ILB-M52W7) is more than 95% and the molecular weight of this protein is around 50-70 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Mouse IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free (Cat. No. ILB-M52W7) at 2 μg/mL (100 μL/well) can bind Human IL-12 R beta 1, Fc Tag (Cat. No. ILB-H5255) with a linear range of 10-312 ng/mL (QC tested).
Protocol
Immobilized Human IL-23 R, Fc Tag (Cat. No. ILR-H5254) at 10 μg/mL (100 μL/well) can bind Mouse IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free (Cat. No. ILB-M52W7) with a linear range of 0.039-0.313 μg/mL (Routinely tested).
Protocol
活性（Bioactivity）-BLI
Loaded Mouse IL-23R, Fc Tag (Cat. No. ILR-M5251) on Protein A Biosensor, can bind Mouse IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free (Cat. No. ILB-M52W7) with an affinity constant of 20.6 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Human IL-23 R, Fc Tag (Cat. No. ILR-H5254) on Protein A Biosensor, can bind Mouse IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free (Cat. No. ILB-M52W7) with an affinity constant of 11.8 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Human IL-12 R beta 1, Fc Tag (Cat. No. ILB-H5255) on Protein A Biosensor, can bind Mouse IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free (Cat. No. ILB-M52W7) with an affinity constant of 3.6 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
活性（Bioactivity）-CELL BASE
Mouse IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free (Cat. No. ILB-M52W7) stimulates secretion of IL-17 by Mouse spleen cells. The ED50 for this effect is 0.008-0.0134 ng/mL (Routinely tested).
Protocol
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