Rat IL-4 R alpha & IL-13 R alpha 1 Protein, His Tag&His Tag (SPR verified)

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ILR-R52H5-100ug
¥4410.00
ILR-R52H5-1mg (500ug X 2)
¥33600.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    IL-4 R alpha & IL-13 R alpha 1, IL4RA & IL13RA1

  • 表达区间及表达系统(Source)

    Rat IL-4 R alpha & IL-13 R alpha 1 Protein, His Tag&His Tag (ILR-R52H5) is expressed from human 293 cells (HEK293). It contains AA Ile 26 - Arg 232 & Thr 27 - Thr 342 (Accession # Q63257-1 & Q561K3).

    Predicted N-terminus: Ile 26 & Thr 27

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    IL-4 R alpha & IL-13 R alpha 1 Structure

    Rat IL-4 R alpha & IL-13 R alpha 1 Protein, His Tag&His Tag, produced by co-expression of IL-4 R alpha and IL-13 R alpha 1, has a calculated MW of 29.1 kDa (IL-4 R alpha) and 41 kDa (IL-13 R alpha 1). Subunit IL-4 R alpha is fused with a polyhistidine tag at the C-terminus, and subunit IL-13 R alpha 1 contains a polyhistidine tag at the C-terminus. The protein migrates as 43-53 kDa and 60-70 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 纯度(Purity)

    >90% as determined by SDS-PAGE.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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数据展示

  • 电泳(SDS-PAGE)

    IL-4 R alpha & IL-13 R alpha 1 SDS-PAGE

    Rat IL-4 R alpha & IL-13 R alpha 1 Protein, His Tag&His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • 活性(Bioactivity)-SPR

     IL-4 R alpha & IL-13 R alpha 1 SPR

    Mouse IL-4, His Tag (Cat. No. IL4-M52H5) immobilized on CM5 Chip can bind Rat IL-4 R alpha & IL-13 R alpha 1 Protein, His Tag&His Tag (Cat. No. ILR-R52H5) with an affinity constant of 8.80 nM as determined in a SPR assay (Biacore 8K) (QC tested).

    Protocol

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背景介绍

Interleukin 4 Receptor (IL4R) also known as CD124, IL4Rα and BSF receptor, is a type I cytokine receptor produced by activated Th2 cells and mast cells, and plays an important role in Th2-­biased immune responses, alternative macrophage activation, mucosal immunity, allergic inflammation, tumor progression, and atherogenesis. A soluble form of the encoded IL4R protein can be produced by an alternate splice variant or by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. IL4R can alternatively associate with IL­13Ra1 to form the type II receptor which is responsive to both IL­4 and IL­13. Interleukin-4 receptor has been shown to interact with SHC1.

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