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分子别名（Synonym）
IL3R, IL3RA, IL-3Ra, IL-3R-alpha, IL3RAY, IL3RX, IL3RY, CD123 antigen, CD123, hIL3Ra, hIL-3Ra, MGC34174, IL-3 R alpha
表达区间及表达系统（Source）
Biotinylated Human IL-3 R alpha, Fc,Avitag (ILA-H82F3) is expressed from human 293 cells (HEK293). It contains AA Thr 19 - Arg 305 (Accession # P26951-1).
Predicted N-terminus: Thr 19
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 61.2 kDa. The protein migrates as 80-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
纯度（Purity）
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
Biotinylated Human IL-3 R alpha, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Biotinylated Human IL-3 R alpha, Fc,Avitag (Cat. No. ILA-H82F3) is more than 95% and the molecular weight of this protein is around 128-156 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Anti-IL-3 R alpha Antibody, Human IgG1 at 1 μg/mL (100 μL/well) can bind Biotinylated Human IL-3 R alpha, Fc,Avitag (Cat. No. ILA-H82F3) with a linear range of 1-20 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Human IL-3 R alpha, Fc,Avitag (Cat. No. ILA-H82F3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-IL-3 R alpha Antibody, Human IgG1 with a linear range of 0.6-5 ng/mL (Routinely tested).
Protocol
活性（Bioactivity）-SPR
Biotinylated Human IL-3 R alpha, Fc,Avitag (Cat. No. ILA-H82F3) immobilized on SA Chip can bind Human IL-3 Protein, His Tag (Cat. No. IL3-H52H9) with an affinity constant of 0.821 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
活性（Bioactivity）-BLI
Loaded Biotinylated Human IL-3 R alpha, Fc,Avitag (Cat. No. ILA-H82F3) on SA Biosensor, can bind Human IL-3 Protein, His Tag (Cat. No. IL3-H52H9) with an affinity constant of 0.35 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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