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Human IL-27 Protein, His Tag, premium grade (Liquid)

COADS MS
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IL7-H5328-100ug
¥3675.00
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产品详情

  • 分子别名（Synonym）

    Interleukin-27, IL27A&IL27B, EBI3&p28

  • 表达区间及表达系统（Source）

    Human IL-27 Protein, His Tag, premium grade (Liquid) (IL7-H5328) is expressed from CHO cells. It contains AA Phe 29 - Pro 243 & Arg 21 - Lys 229 (Accession # Q14213-1 & Q8NEV9-1).

    Predicted N-terminus: Phe 29

    It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.

  • 蛋白结构（Molecular Characterization）

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 48.6 kDa. The protein migrates as 52 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 内毒素（Endotoxin）

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • 宿主核酸残留（Host Cell DNA）

    <0.02 ng/μg of protein tested by qPCR.

  • 无菌（Sterility）

    Negative

  • 支原体（Mycoplasma）

    Negative

  • 纯度（Purity）

    >95% as determined by SDS-PAGE.

  • 制剂（Formulation）

    Supplied as 0.2 μm filtered solution in PBS, pH7.4 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • 运输（Shipping）

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • 存储（Storage）

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.

  • 质量管理控制体系（QMS）

    1. 质量管理体系（ISO, GMP）
    2. 质量优势
    3. 质控流程

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数据展示

  • 电泳（SDS-PAGE）

    IL-27 SDS-PAGE

    Human IL-27 Protein, His Tag, premium grade (Liquid) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • 活性（Bioactivity）-CELL BASE

    IL-27 CELL

    Human IL-27 Protein, His Tag, premium grade (Liquid) (Cat. No. IL7-H5328) induced STAT3 (Luc) Reporter activity in HEK293 cells. The EC50 for this effect is 0.1175 μg/mL (Routinely tested).

    Protocol

  • 活性（Bioactivity）-ELISA

    IL-27 ELISA

    Immobilized Human IL-27 Protein, His Tag, premium grade (Liquid) (Cat. No. IL7-H5328) at 2 μg/mL (100 μL/well) can bind Human IL-27 Ra, Fc Tag (Cat. No. ILA-H5254) with a linear range of 0.001-0.125 μg/mL (QC tested).

    Protocol

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背景介绍

Interleukin-27 (IL-27) is a heterodimeric cytokine belonging to the IL-12 family that is composed of two subunits, Epstein-Barr virus (EBV)-induced gene 3 (EBI3) (also known as IL-27B) and IL27-p28 (known as IL-30). IL-27 is produced by antigen-presenting cells. IL-27 plays an important function in regulating the activity of B and T lymphocytes. The effects of IL-27 are eliciting by its interaction with a specific cell surface receptor complex composed of two proteins known as IL27R and gp130. IL-27 is a cytokine with pro- and anti-inflammatory properties, that can regulate T helper cell development, suppress T-cell proliferation, stimulate cytotoxic T cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. IL-27 reveals to be a potent inhibitor of TH17 cell development and of IL-17 production. IL-27 also antagonizes the effects of some cytokines such as IL6 through direct effects on T cells. Another important role of IL-27 is its antitumor activity and antiangiogenic activity.

前沿进展

 
药物研发进展
  • 英文全称：

    Interleukin-27

  • 中文全称：

    白细胞介素-27

  • 种类：

  • 上市药物数量：

    0 详情

  • 临床药物数量：

    1 详情

  • 最高研发阶段：

    临床二期

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