Human IL-21 R & IL-2 R gamma Heterodimer Protein, Fc Tag&Fc Tag (MALS verified)

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ILA-H52W9-100ug
¥3675.00
ILA-H52W9-1mg
¥24255.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    IL-21 R & IL-2 R gamma,IL21R & IL2RG,IL-21R & IL-2RG

  • 表达区间及表达系统(Source)

    Human IL-21 R & IL-2 R gamma Heterodimer Protein, Fc Tag&Fc Tag (ILA-H52W9) is expressed from human 293 cells (HEK293). It contains AA Cys 20 - Pro 236 (IL-21 R) & Leu 23 - Asn 254 (IL-2 R gamma) (Accession # Q9HBE5-1 (IL-21 R) & P31785-1 (IL-2 R gamma)).

    Predicted N-terminus: Cys 20 (IL-21 R) & Leu 23 (IL-2 R gamma)

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    IL-21 R & IL-2 R gamma Structure

    Human IL-21 R & IL-2 R gamma Heterodimer Protein, Fc Tag&Fc Tag is produced by co-expression of IL-21 R and IL-2 R gamma, has a calculated MW of 51.5 kDa (IL-21 R) & 53.6 kDa (IL-2 R gamma). Subunit IL-21 R is fused with a human IgG1 Fc tag at the C-terminus and subunit IL-2 R gamma is fused with a human IgG1 Fc tag at the C-terminus. The protein migrates as 65 kDa and 70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 内毒素(Endotoxin)

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • 纯度(Purity)

    >95% as determined by SDS-PAGE.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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数据展示

  • 电泳(SDS-PAGE)

    IL-21 R & IL-2 R gamma SDS-PAGE

    Human IL-21 R & IL-2 R gamma Heterodimer Protein, Fc Tag&Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    IL-21 R & IL-2 R gamma SEC-MALS

    The purity of Human IL-21 R & IL-2 R gamma Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. ILA-H52W9) is more than 85% and the molecular weight of this protein is around 128-156 kDa verified by SEC-MALS.

    Report
  • 活性(Bioactivity)-ELISA

     IL-21 R & IL-2 R gamma ELISA

    Immobilized Human IL-21, Tag Free (Cat. No. IL1-H5213) at 5 μg/mL (100 μL/well) can bind Human IL-21 R & IL-2 R gamma Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. ILA-H52W9) with a linear range of 5-78 ng/mL (QC tested).

    Protocol

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背景介绍

Interleukin (IL)-21, IL-2, IL-4, IL-7, IL-9, and IL-15 form a family of cytokines based on their sharing the common cytokine receptor γ chain (γc), which was originally discovered as the third receptor component of the IL-2 receptor,IL-2RG. IL-21 is a class I cytokine that exerts pleiotropic effects on both innate and adaptive immune responses. It signals through a heterodimeric receptor complex consisting of the IL-21 receptor (IL-21R) and the common γ-chain.

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