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分子别名（Synonym）
IL21, Za11, Interleukin-21
产品描述（Product Details）
Our Recombinant Human IL-21 Superior Stable Mutant Protein, developed via an AI-modified and experimentally validated closed-loop efficient protein design platform, is the optimal IL-21 mutant with superior thermal stability and activity compared to wild-type IL-21, making it more suitable for various immune cell culture processes. This reduces medium replacement frequency, lowers contamination risks from frequent operations, and saves costs/time for immune cell culture by minimizing reagent consumption and labor, especially in large-scale applications.
This product is designed and developed utilizing our proprietary AI technology, which is protected by patent rights. We retain full commercial rights to this product.It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.
蛋白结构（Molecular Characterization）
This protein carries no "tag".
The protein has a calculated MW of 15.6 kDa. The protein migrates as 16 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
内毒素（Endotoxin）
Less than 0.01 EU per μg by the LAL method / rFC method.
宿主蛋白残留（Host Cell Protein）
<0.5 ng/µg of protein tested by ELISA.
宿主核酸残留（Host Cell DNA）
<0.02 ng/μg of protein tested by qPCR.
无菌（Sterility）
Negative
支原体（Mycoplasma）
Negative
纯度（Purity）
>95% as determined by SDS-PAGE.
>95% as determined by SEC-HPLC.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
Human IL-21 Superior Stable Mutant Protein, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-HPLC
The purity of Human IL-21 Superior Stable Mutant Protein, premium grade (Cat. No. IL1-H5113) was greater than 95% as determined by SEC-HPLC.
热稳定性（Heat Stability）
Recombinant Human IL-21 stability studies in the T culture medium. Human IL-21 Superior Stable Mutant Protein, premium grade (Cat. No. IL1-H5113) or wild-type (WT) Human IL-21 Protein, premium grade (Cat. No. IL1-H5114), or Company M Human IL-21 Protein were added in the T cell medium at an initial concentration of 22 ng/mL, respectively. IL-21 concentration tested with resDetect™ Human Interleukin-21 (IL-21) ELISA Kit (Cat. No. CRS-A010). Following 3 days of treatment at 37°C, Recombinant Human IL-21 Superior Stable Mutant Protein retained the concentration 70%, demonstrating its enhanced thermal stability. In contrast, the WT Recombinant Human IL-21 Protein exhibited significant concentration loss, indicating inferior thermal stability.
活性（Bioactivity）-CELL BASE
Human IL-21 Superior Stable Mutant Protein, premium grade (Cat. No. IL1-H5113) stimulates secretion of IFN-γ by NK-92 human natural killer lymphoma cells stimulated with Human IL-15, premium grade (Cat. No. IL5-H4117), The specific activity of Human IL-21 Superior Stable Mutant Protein, premium grade is > 4.00 x 10^5 U/mg (QC tested).
Protocol
活性（Bioactivity）-ELISA
Immobilized Human IL-21 Superior Stable Mutant Protein, premium grade (Cat. No. IL1-H5113) at 5 μg/mL (100 μL/well) can bind Human IL-21 R, Fc Tag with a linear range of 0.5-8 ng/mL (QC tested).
Protocol
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