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分子别名（Synonym）
IL2RA, CD25, p55, IL2-RA, IL-2-RA
表达区间及表达系统（Source）
Biotinylated Cynomolgus IL-2 R alpha Protein, His,Avitag (ILA-C82E4) is expressed from human 293 cells (HEK293). It contains AA Glu 22 - Glu 238 (Accession # H6WS54).
Predicted N-terminus: Glu 22
Request for sequence蛋白结构（Molecular Characterization）
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 28.1 kDa. The protein migrates as 50-60 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
纯度（Purity）
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
Biotinylated Cynomolgus IL-2 R alpha Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Cynomolgus IL-2 R alpha Protein, His,Avitag (Cat. No. ILA-C82E4) is more than 90% and the molecular weight of this protein is around 48-65 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Cynomolgus IL-2, His Tag (Cat. No. IL2-C5249) at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus IL-2 R alpha Protein, His,Avitag (Cat. No. ILA-C82E4) with a linear range of 0.01-1.25 μg/mL (QC tested).
Protocol
Immobilized Biotinylated Cynomolgus IL-2 R alpha Protein, His,Avitag (Cat. No. ILA-C82E4) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human IL-2 Protein, Fc Tag (Cat. No. IL2-H5269) with a linear range of 0.2-4 ng/mL (Routinely tested).
Protocol
活性（Bioactivity）-SPR
Human IL-2 Protein, Tag Free (Cat. No. IL2-H5215) immobilized on CM5 Chip can bind Biotinylated Cynomolgus IL-2 R alpha Protein, His,Avitag (Cat. No. ILA-C82E4) with an affinity constant of 103 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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