产品详情
分子别名(Synonym)
IL18BP, IL18BPa, Tadekinig-alfa
表达区间及表达系统(Source)
Human IL-18BP, Fc Tag (ILP-H5253) is expressed from human 293 cells (HEK293). It contains AA Thr 31 - Gly 194 (Accession # NP_001138529).
Predicted N-terminus: Thr 31
Request for sequence蛋白结构(Molecular Characterization)

This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 44.3 kDa. The protein migrates as 55-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method / rFC method.
纯度(Purity)
>95% as determined by SDS-PAGE.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系(QMS)
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数据展示
电泳(SDS-PAGE)

Human IL-18BP, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS

The purity of Human IL-18BP, Fc Tag (Cat. No. ILP-H5253) is more than 85% and the molecular weight of this protein is around 105-130 kDa verified by SEC-MALS.
Report
活性(Bioactivity)-ELISA

Immobilized Human IL-18 Protein, premium grade (Cat. No. IL8-H5114) at 5 μg/mL (100 μL/well) can bind Human IL-18BP, Fc Tag (Cat. No. ILP-H5253) with a linear range of 0.6-20 ng/mL (QC tested).
Protocol
活性(Bioactivity)-SPR

Human IL-18BP, Fc Tag (Cat. No. ILP-H5253) captured on Protein A Chip can bind Human IL-18 Protein, premium grade (Cat. No. IL8-H5114) with an affinity constant of 0.781 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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