Biotinylated Human IL-18 R1 / CD218a Protein, His,Avitag™ (MALS & SPR verified)

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IL1-H82E7-25ug
¥3675.00
IL1-H82E7-200ug
¥14490.00
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产品详情

  • 分子别名(Synonym)

    IL18R1, IL1RRP, CD218a, IL18Ra

  • 表达区间及表达系统(Source)

    Biotinylated Human IL-18 R1, His,Avitag (IL1-H82E7) is expressed from human 293 cells (HEK293). It contains AA Ala 19 - Arg 329 (Accession # Q13478-1).

    Predicted N-terminus: Ala 19

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    IL-18 R1 Structure

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 39.3 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 标记(Labeling)

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • 蛋白标记度(Protein Ratio)

    Passed as determined by the HABA assay / binding ELISA.

  • 纯度(Purity)

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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数据展示

  • 电泳(SDS-PAGE)

    IL-18 R1 SDS-PAGE

    Biotinylated Human IL-18 R1, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    IL-18 R1 SEC-MALS

    The purity of Biotinylated Human IL-18 R1, His,Avitag (Cat. No. IL1-H82E7) is more than 95% and the molecular weight of this protein is around 65-75 kDa verified by SEC-MALS.

    Report
  • 活性(Bioactivity)-SPR

     IL-18 R1 SPR

    Human IL-18 R1, His,Avitag (Cat. No. IL1-H82E7) immobilized on SA Chip can bind Human IL-18 Protein, premium grade (Cat. No. IL8-H5114) with an affinity constant of 3.36 nM as determined in a SPR assay (Biacore 8K) (QC tested).

    Protocol

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背景介绍

Interleukin-18 receptor 1 (IL18R1) is also known as CD218 antigen-like family member A (CD218a), IL1 receptor-related protein (IL-1Rrp or IL1R-rp), CDw218a, IL18Ra, IL1RRP, which belongs to the interleukin-1 receptor family. IL18R1 contains three Ig-like C2-type (immunoglobulin-like) domains and one TIR domain. IL18R1 is receptor for interleukin 18 (IL-18). IL18R1 binds to the agonist leads to the activation of NF-kappa-B, but does not bind IL1A/interleukin-1 alpha or IL1B/interleukin-1 beta.

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  • 英文全称:

    Interleukin-18 receptor 1

  • 中文全称:

    白细胞介素18受体1

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