Biotinylated Human IL-17B Protein, Fc,Avitag™ (MALS verified)

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Qty.
ILB-H82F1-25ug
¥3675.00
ILB-H82F1-200ug
¥14490.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    Interleukin-17B,Cytokine Zcyto7,Interleukin-20,IL-20,Neuronal interleukin-17-related factor,IL17B,IL20, NIRF, ZCYTO7

  • 表达区间及表达系统(Source)

    Biotinylated Human IL-17B, Fc,Avitag (ILB-H82F1) is expressed from human 293 cells (HEK293). It contains AA Gln 21 - Phe 180 (Accession # Q9UHF5-1).

    Predicted N-terminus: Gln 21

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    IL-17B Structure

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 45.8 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 标记(Labeling)

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • 蛋白标记度(Protein Ratio)

    Passed as determined by the HABA assay / binding ELISA.

  • 纯度(Purity)

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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数据展示

  • 电泳(SDS-PAGE)

    IL-17B SDS-PAGE

    Biotinylated Human IL-17B, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    IL-17B SEC-MALS

    The purity of Biotinylated Human IL-17B, Fc,Avitag (Cat. No. ILB-H82F1) is more than 90% and the molecular weight of this protein is around 100-120 kDa verified by SEC-MALS.

    Report
  • 活性(Bioactivity)-ELISA

     IL-17B ELISA

    Immobilized Biotinylated Human IL-17B, Fc,Avitag (Cat. No. ILB-H82F1) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-Human IL17B Monoclonal Antibody with a linear range of 0.1-8 ng/mL (Routinely tested).

    Protocol

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背景介绍

IL-17B is a member of IL17 famaily, was originally described as a proinflammatory cytokine. IL-17B is produced by neutrophils and potentiates TNF-α effect on the production of cytokines and chemokines, such as IL-6, G-CSF, and CCL20, known to control immune cell trafficking to inflamed tissues. Interestingly, although IL-17B and IL-17E (IL-25) share a common receptor, IL-17RB, IL-17B, and IL-17E deficiency lead to opposite results in a model of acute colitis induced by dextran sulfate sodium. IL-17B inhibits IL-17E binding to IL-17RA– IL-17RB complexes on epithelial cells, and limits IL-17E-induced IL-6 production by colon epithelial cells.

前沿进展

 
药物研发进展
  • 英文全称:

    Interleukin-17 receptor B

  • 中文全称:

    白细胞介素-17受体B

  • 种类:

  • 上市药物数量:

    0 详情

  • 临床药物数量:

    1 详情

  • 最高研发阶段:

    临床一期

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