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分子别名（Synonym）
CD217, CDw217, IL-17RA, IL17R, CANDF5, hIL-17R
表达区间及表达系统（Source）
Biotinylated Human IL-17 RA, Fc,Avitag (ILA-H82F1) is expressed from human 293 cells (HEK293). It contains AA Leu 33 - Trp 320 (Accession # Q96F46-1).
Predicted N-terminus: Leu 33
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 61.9 kDa. The protein migrates as 80-95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
纯度（Purity）
>95% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
Biotinylated Human IL-17 RA, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-ELISA
Immobilized Human IL17A, premium grade (Cat. No. ILA-H5219) at 2 μg/mL (100 μL/well) can bind Biotinylated Human IL-17 RA, Fc,Avitag (Cat. No. ILA-H82F1) with a linear range of 2-125 ng/mL (QC tested).
Protocol
Immobilized Human IL-17F (H161R), His Tag (Cat. No. ILF-H4240) at 10 μg/mL (100 μL/well) can bind Biotinylated Human IL-17 RA, Fc,Avitag (Cat. No. ILA-H82F1) with a linear range of 0.02-0.313 μg/mL (Routinely tested).
Protocol
活性（Bioactivity）-BLI
Loaded Biotinylated Human IL-17 RA, Fc,Avitag (Cat. No. ILA-H82F1) on SA Biosensor, can bind Human IL17A, Tag Free with an affinity constant of 1.29 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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