FITC-Labeled Human IL-15 R alpha / CD215 Protein, Fc Tag (MALS verified)

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ILA-HF255-25ug
¥3350.00
ILA-HF255-200ug
¥20790.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    IL-15 R alpha, CD215, IL15RA, IL-15RA, IL-15R-alpha

  • 表达区间及表达系统(Source)

    FITC-Labeled Human IL-15 R alpha, Fc Tag (ILA-HF255) is expressed from human 293 cells (HEK293). It contains AA Ile 31 - Thr 205 (Accession # Q13261-1). It is the FITC labeled form of Human IL-15 R alpha, Fc Tag (Cat. No. ILA-H5253).

    Predicted N-terminus: Ile 31

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    IL-15 R alpha Structure

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 44.8 kDa. The protein migrates as 65-80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 偶联(Conjugate)

    FITC

    Excitation source: 488 nm spectral line, argon-ion laser

    Excitation Wavelength: 488 nm

    Emission Wavelength: 535 nm

  • 标记(Labeling)

    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.

  • 蛋白标记度(Protein Ratio)

    The FITC to protein molar ratio is 1.5-3.5.

  • 纯度(Purity)

    >95% as determined by SDS-PAGE.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, 0.2 M Arginine, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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数据展示

  • 电泳(SDS-PAGE)

    IL-15 R alpha SDS-PAGE

    FITC-Labeled Human IL-15 R alpha, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    IL-15 R alpha SEC-MALS

    The purity of FITC-Labeled Human IL-15 R alpha, Fc Tag (Cat. No. ILA-HF255) is more than 85% and the molecular weight of this protein is around 115-140 kDa verified by SEC-MALS.

    Report
  • 活性(Bioactivity)-ELISA

     IL-15 R alpha ELISA

    Immobilized Human IL-15, His Tag (Cat. No. IL5-H52H8) at 2 μg/mL (100 μL/well) can bind FITC-Labeled Human IL-15 R alpha, Fc Tag (Cat. No. ILA-HF255) with a linear range of 0.078-5 μg/mL (QC tested).

    Protocol

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背景介绍

IL15R is a heterotrimer of IL15RA, IL2RB and IL2RG. IL-15RA, also known as CD215, IL-15 R alpha, is a widely expressed 60 kDa transmembrane glycoprotein. High-affinity receptor for interleukin-15. Can signal both in cis and trans where IL15R from one subset of cells presents IL15 to neighboring IL2RG-expressing cells. Expression of different isoforms may alter or interfere with signal transduction. An interleukin (IL)-15 superagonist/IL-15 receptor α fusion complex (IL-15SA/IL-15RA; ALT-803) activates the IL-15 receptor on CD8 T cells and NK cells, and has shown significant anti-tumor. Signal transduction involves SYK. Higher levels of soluble sIL-15RA form in comparison with membrane-bound forms is present in all brain structures.

前沿进展

 
药物研发进展
  • 英文全称:

    Interleukin-15 receptor subunit alpha

  • 中文全称:

    白细胞介素-15受体α亚基

  • 种类:

  • 上市药物数量:

    0 详情

  • 临床药物数量:

    13 详情

  • 最高研发阶段:

    临床二期

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