Biotinylated Human IL-10 Protein, His,Avitag™

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Qty.
IL0-H82Q3-25ug
¥3675.00
IL0-H82Q3-200ug
¥14490.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    CSIF,IL-10,IL10A,TGIF,B-TCGF,GVHDS,MGC126450,MGC126451,RP11-262N9.1,Interleukin-10

  • 表达区间及表达系统(Source)

    Biotinylated Human IL-10 Protein, His,Avitag (IL0-H82Q3) is expressed from human 293 cells (HEK293). It contains AA Ser 19 - Asn 178 (Accession # NP_000563.1).

    Predicted N-terminus: His

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    IL-10 Structure

    This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 22.2 kDa. The protein migrates as 25-28 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 标记(Labeling)

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • 蛋白标记度(Protein Ratio)

    Passed as determined by the HABA assay / binding ELISA.

  • 内毒素(Endotoxin)

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • 纯度(Purity)

    >90% as determined by SDS-PAGE.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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  • 电泳(SDS-PAGE)

    IL-10 SDS-PAGE

    Biotinylated Human IL-10 Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • 活性(Bioactivity)-ELISA

     IL-10 ELISA

    Immobilized Biotinylated Human IL-10 Protein, His,Avitag (Cat. No. IL0-H82Q3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human IL-10 R alpha Protein, Fc Tag (Cat. No. ILR-H525a) with a linear range of 0.05-6 ng/mL (QC tested).

    Protocol

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背景介绍

The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. [provided by RefSeq, May 2020]

前沿进展

 
药物研发进展
  • 英文全称:

    Interleukin-10

  • 中文全称:

    白细胞介素-10

  • 种类:

  • 上市药物数量:

    0 详情

  • 临床药物数量:

    6 详情

  • 最高研发阶段:

    临床三期

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