Rat IGF-I R / IGF-1 R Protein, His Tag (MALS verified)

用户评价
货号-规格
价格
Qty.
IGR-R5224-100ug
¥3675.00
IGR-R5224-1mg (500ug X 2)
¥24255.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    IGF-I R / IGF-1 R

  • 表达区间及表达系统(Source)

    Rat IGF-I R, His Tag (IGR-R5224) is expressed from human 293 cells (HEK293). It contains AA Glu 31 - His 936 (Accession # P24062-1).

    Predicted N-terminus: Glu 31

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    IGF-I R / IGF-1 R Structure

    This protein carries a polyhistidine tag at the C-terminus. This protein contains a furin convertase cleavage site, 738-RRRR-741, and will be partially processed into N (α chain) and C-terminal fragment (partial β chain) with calculated MW of 81.3 kDa and 24.2 kDa respectively. The protein migrates as 45-50 kDa (partial β chain), 96-115 kDa (α chain) and 120 kDa (α chain & partial β chain) under reducing (R) condition (SDS-PAGE) due to glycosylation.

    The protein is designed as a dimer.

  • 内毒素(Endotoxin)

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • 纯度(Purity)

    >90% as determined by SDS-PAGE.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

推荐产品

数据展示

  • 电泳(SDS-PAGE)

    IGF-I R / IGF-1 R SDS-PAGE

    Rat IGF-I R, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • SEC-MALS

    IGF-I R / IGF-1 R SEC-MALS

    The purity of Rat IGF-I R, His Tag (Cat. No. IGR-R5224) is more than 85% and the molecular weight of this protein is around 254-310 kDa verified by SEC-MALS.

    Report
  • 活性(Bioactivity)-ELISA

     IGF-I R / IGF-1 R ELISA

    Immobilized Rat IGF-I R, His Tag (Cat. No. IGR-R5224) at 5 μg/mL (100 μL/well) can bind Human IGF-I, Fc Tag with a linear range of 5-39 ng/mL (QC tested).

    Protocol

用户评价
发表评论

背景介绍

The Insulin-like Growth Factor 1 Receptor (IGF1) is also known as CD221, JTK13. and is a transmembrane receptor that is activated by IGF-1 and by the related growth factor IGF-2. It belongs to the large class of tyrosine kinase receptors. This receptor mediates the effects of IGF-1, which is a polypeptide protein hormone similar in molecular structure to insulin. IGF1R is make up of two alpha subunits and two beta subunits ,the Both the α and β subunits are synthesized from a single mRNA precursor. The precursor is then glycosylated, proteolytically cleaved, and crosslinked by cysteine bonds to form a functional transmembrane αβ chain.The α chains are located extracellularly while the β subunit spans the membrane and are responsible for intracellular signal transduction upon ligand stimulation. IGF1R have a binding site for ATP, which is used to provide the phosphates for autophosphorylation. There is a 60% homology between IGF1R and the insulin receptor. In response to ligand binding, the α chains induce the tyrosine autophosphorylation of the β chains. This event triggers a cascade of intracellular signaling that, while somewhat cell type specific, often promotes cell survival and cell proliferation.

前沿进展

 
联系我们
项目合作
查看更多项目
实验案例分析
查看更多案例分析
  • 产品基础信息
  • 产品详情
  • 推荐产品
  • 数据展示
  • 用户评价
  • 背景介绍