Human IDO1 Protein, His Tag (active enzyme, MALS verified)

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ID1-H5143-100ug
¥3675.00
ID1-H5143-1mg
¥26250.00
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产品详情

  • 分子别名(Synonym)

    IDO, IDO-1, INDO

  • 表达区间及表达系统(Source)

    Human IDO1 Protein, His Tag (ID1-H5143) is expressed from E. coli cells. It contains AA Ala 2 - Gly 403 (Accession # P14902-1).

    Predicted N-terminus: Met

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    IDO1 Structure

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 47.2 kDa. The protein migrates as 45-50 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

  • 内毒素(Endotoxin)

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • 纯度(Purity)

    >90% as determined by SDS-PAGE.

  • 制剂(Formulation)

    Supplied as 0.2 μm filtered solution in 20 mM Tris, 300 mM NaCl, pH7.5 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • 运输(Shipping)

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • 存储(Storage)

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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数据展示

  • 电泳(SDS-PAGE)

    IDO1 SDS-PAGE

    Human IDO1 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    IDO1 SEC-MALS

    The purity of Human IDO1 Protein, His Tag (Cat. No. ID1-H5143) is more than 85% and the molecular weight of this protein is around 40-60 kDa verified by SEC-MALS.

    Report
  • 活性(Bioactivity)

    Measured by its ability to oxidize L-tryptophan to N-formyl-kynurenine. The specific activity is >400 pmol/min/μg (QC tested).

    Protocol

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背景介绍

This gene encodes indoleamine 2,3-dioxygenase (IDO) - a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan.[provided by RefSeq, Feb 2011]

前沿进展

 
药物研发进展
  • 英文全称:

    Indoleamine 2,3-dioxygenase

  • 中文全称:

    吲哚胺2,3-双加氧酶

  • 种类:

  • 上市药物数量:

    0 详情

  • 临床药物数量:

    11 详情

  • 最高研发阶段:

    临床三期

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