Human IDH1 Protein, His Tag (active enzyme, MALS verified)

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ID1-H51H6-20ug
¥3675.00
ID1-H51H6-100ug
¥10290.00
ID1-H51H6-500ug
¥36750.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    IDH1, PICD, IDP

  • 表达区间及表达系统(Source)

    Human IDH1, His Tag (ID1-H51H6) is expressed from E. coli cells. It contains AA Met 1 - Leu 414 (Accession # O75874-1).

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    IDH1 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 48.5 kDa. The protein migrates as 45-50 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

    The protein is designed as a dimer.

  • 纯度(Purity)

    >95% as determined by SDS-PAGE.

  • 制剂(Formulation)

    Supplied as 0.2 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • 运输(Shipping)

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • 存储(Storage)

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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数据展示

  • 电泳(SDS-PAGE)

    IDH1 SDS-PAGE

    Human IDH1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    IDH1 SEC-MALS

    The purity of Human IDH1, His Tag (Cat. No. ID1-H51H6) is more than 85% and the molecular weight of this protein is around 90-100 kDa verified by SEC-MALS.

    Report
  • 活性(Bioactivity)

    Measured by the ability to oxidatively decarboxylate isocitrate to 2-oxoglutarate. The specific activity is >10000 pmol/min/μg, as measured under the described conditions (QC tested).

    Protocol

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背景介绍

The IDH enzymes catalyze the oxidative decarboxylation of isocitrate to alpha-ketoglutarate (α-KG), producing nicotinamide adenine dinucleotide phosphate (NADPH) in the process via the citric acid cycle. Eukaryotic cells express two distinct classes of IDHs that utilize either NAD or NADP as their cofactors and serve diverse biological functions. NAD-dependent IDH is localized to the mitochondrial matrix and is well known for its central role for energy production in the Krebs cycle. NADP-dependent IDHs are primarily located either in mitochondria or cytoplasm . Each NADP-dependent isozyme is a homodimer. Mutations of Arg132 of human IDH1 result in a reduced ability of the enzyme to convert isocitrate to alpha ‑ketoglutarate, but the enzyme acquires the ability to generate 2-hydroxyglutarate (2HG) from alpha‑ketoglutarate,2-HG is elevated in several tumor types, including a subset of AMLs.

前沿进展

 
药物研发进展
  • 英文全称:

    Isocitrate dehydrogenase [NADP] cytoplasmic

  • 中文全称:

    细胞质异柠檬酸脱氢酶[NADP]

  • 种类:

  • 上市药物数量:

    3 详情

  • 临床药物数量:

    11 详情

  • 最高研发阶段:

    批准上市

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