Biotinylated Human HLA-DRA1*01:01&HLA-DRB1*04:01&Influenza HA (PKYVKQNTLKLAT) Complex Protein (Monomer)

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HLA-H82Eb-25ug
¥1260.00
HLA-H82Eb-200ug
¥7140.00
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产品详情

  • 分子别名(Synonym)

    HLA-DRA1*0101 & HLA-DRB1*0401 & Influenza HA (PKYVKQNTLKLAT)

  • 表达区间及表达系统(Source)

    Biotinylated Human HLA-DRA1*01:01&HLA-DRB1*04:01&Influenza HA (PKYVKQNTLKLAT) Complex Protein (HLA-H82Eb) is expressed from human 293 cells (HEK293). It contains AA Ile 26 - Glu 216 (HLA-DRA1*01:01) & Gly 30 - Lys 227 (HLA-DRB1*04:01) & PKYVKQNTLKLAT peptide (Accession # CAI2388006.1 (HLA-DRA1*01:01) & CAI9239961.1 (HLA-DRB1*04:01) & PKYVKQNTLKLAT).

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  • 蛋白结构(Molecular Characterization)

    Biotinylated Human HLA-DRA1*01:01&HLA-DRB1*04:01&Influenza HA (PKYVKQNTLKLAT) Complex Protein is produced by co-expression of HLA-DRA1 and HLA-DRB1 loaded with Influenza HA peptide.

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 29.8 kDa and 29.0 kDa. The protein migrates as 33-40 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 标记(Labeling)

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • 纯度(Purity)

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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数据展示

  • 电泳(SDS-PAGE)

    HLA-DRA1*0101 & HLA-DRB1*0401 & Influenza HA (PKYVKQNTLKLAT) SDS-PAGE

    Biotinylated Human HLA-DRA1*01:01&HLA-DRB1*04:01&Influenza HA (PKYVKQNTLKLAT) Complex Protein on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    HLA-DRA1*0101 & HLA-DRB1*0401 & Influenza HA (PKYVKQNTLKLAT) SEC-MALS

    The purity of Biotinylated Human HLA-DRA1*01:01&HLA-DRB1*04:01&Influenza HA (PKYVKQNTLKLAT) Complex Protein (Cat. No. HLA-H82Eb) is more than 90% and the molecular weight of this protein is around 55-75 kDa verified by SEC-MALS.

    Report
  • 活性(Bioactivity)-ELISA

     HLA-DRA1*0101 & HLA-DRB1*0401 & Influenza HA (PKYVKQNTLKLAT) ELISA

    Immobilized Biotinylated Human HLA-DRA1*01:01&HLA-DRB1*04:01&Influenza HA (PKYVKQNTLKLAT) Complex Protein (Cat. No. HLA-H82Eb) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA-DR Antibody, Human IgG1 with a linear range of 0.008-0.2 ng/mL (QC tested).

    Protocol

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背景介绍

The peptide PKYVKQNTLKLAT, derived from the hemagglutinin (HA) protein of influenza virus, is presented by the MHC class II molecule HLA-DRA1*01:01/HLA-DRB1*04:01. This complex serves as a prototypical target for virus-specific CD4⁺ T helper cells, which are crucial for generating effective antibody responses and cytotoxic T lymphocyte (CTL) memory during influenza infection and vaccination. Studying this epitope has been fundamental to understanding adaptive immunity to viral pathogens and HLA-restricted immune recognition.

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