Unconjugated Human HLA-A*33:03&B2M&EGF R (HVKITDFGR) Complex Protein (Monomer, MALS verified)

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HLR-H82Ef-25ug
¥1200.00
HLR-H82Ef-200ug
¥6800.00
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产品详情

  • 分子别名(Synonym)

    HLA-A*3303 & B2M & EGF R (HVKITDFGR)

  • 表达区间及表达系统(Source)

    Unconjugated Human HLA-A*33:03&B2M&EGF R (HVKITDFGR) Complex Protein (HLR-H82Ef) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Ile 308 (HLA-A*33:03) & Ile 21 - Met 119 (B2M) & HVKITDFGR peptide (Accession # AAA79865.1 (HLA-A*33:03) & P61769-1 (B2M) & HVKITDFGR).

    Predicted N-terminus: Gly 25 & His

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    Unconjugated Human HLA-A*33:03&B2M&EGF R (HVKITDFGR) Complex Protein is produced by co-expression of HLA-A*33:03 and B2M loaded with EGF R peptide.

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™), and it is not biotinylated.

    The protein has a calculated MW of 36.2 kDa and 13.8 kDa. The protein migrates as 40-43 kDa and 12 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 纯度(Purity)

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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数据展示

  • 电泳(SDS-PAGE)

    HLA-A*3303 & B2M & EGF R (HVKITDFGR) SDS-PAGE

    Unconjugated Human HLA-A*33:03&B2M&EGF R (HVKITDFGR) Complex Protein on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    HLA-A*3303 & B2M & EGF R (HVKITDFGR) SEC-MALS

    The purity of Unconjugated Human HLA-A*33:03&B2M&EGF R (HVKITDFGR) Complex Protein (Cat. No. HLR-H82Ef) is more than 90% and the molecular weight of this protein is around 45-65 kDa verified by SEC-MALS.

    Report
  • 活性(Bioactivity)-ELISA

     HLA-A*3303 & B2M & EGF R (HVKITDFGR) ELISA

    Immobilized Biotinylated Human HLA-A*33:03 & B2M & EGF R (HVKITDFGR) Complex Protein (Monomer) (Cat. No. HLR-H82Ef) at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-B2M Antibody, Human IgG1 with a linear range of 0.01-1 ng/mL (QC tested).

    Protocol

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背景介绍

The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer. The Human HLA-A*3303 & B2M & EGF R(HVKITDFGR) protein is a complex of HLA-A*3303 of the MHC Class I, B2M, and HVKITDFGR peptide of the EGF R.

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