产品详情
分子别名(Synonym)
ERBB2,CD340,HER-2,neu,HER2,MLN19,NEU,NGL,TKR1
表达区间及表达系统(Source)
Human Her2 (23-510), His Tag (HE2-H52H9) is expressed from human 293 cells (HEK293). It contains AA Thr 23 - Ala 510 (Accession # P04626-1).
Predicted N-terminus: Thr 23
Request for sequence蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 55.6 kDa. The protein migrates as 60-70 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. This protein has been verified not recognized by Trastuzumab in functional ELISA at ACRObiosystems.
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method / rFC method.
纯度(Purity)
>95% as determined by SDS-PAGE.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系(QMS)
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数据展示
电泳(SDS-PAGE)

Human Her2 (23-510), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS

The purity of Human Her2 (23-510), His Tag (Cat. No. HE2-H52H9) is more than 85% and the molecular weight of this protein is around 55-70 kDa verified by SEC-MALS.
Report
活性(Bioactivity)-ELISA

Immobilized Human Her2 (23-510), His Tag (Cat. No. HE2-H52H9) at 1 μg/mL (100 μL/well) can bind Pertuzumab Biosimilar with a linear range of 0.1-4 ng/mL (QC tested).
Protocol
Immobilized Human Her2 (23-510), His Tag (Cat. No. HE2-H52H9) at 1 μg/mL (100 μL/well) can bind Bispecific Antibody (Her2 × Her3) with a linear range of 0.2-3 ng/mL (Routinely tested).
Protocol
Immobilized Human Her2 (23-510), His Tag (Cat. No. HE2-H52H9) at 1 μg/mL (100 μL/well) can bind Pertuzumab with a linear range of 0.2-3 ng/mL,This protein has been verified not recognized by Trastuzumab in functional ELISA at ACRObiosystems. (Routinely tested).
Protocol
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