FITC-Labeled Human GUCY2C / Guanylyl cyclase C Protein, Fc Tag

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GUC-HF255-25ug
¥3350.00
GUC-HF255-200ug
¥20790.00
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产品详情

  • 分子别名(Synonym)

    GUCY2C, GUC2C, STAR, STA receptor, hSTAR, GC-C

  • 表达区间及表达系统(Source)

    FITC-Labeled Human GUCY2C, Fc Tag (GUC-HF255) is expressed from human 293 cells (HEK293). It contains AA Ser 24 - Gln 430 (Accession # P25092-1). It is the FITC labeled form of Human GUCY2C, Fc Tag (Cat. No. GUC-H5257).

    Predicted N-terminus: Ser 24

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    GUCY2C Structure

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 72.5 kDa. The protein migrates as 90-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 偶联(Conjugate)

    FITC

    Excitation source: 488 nm spectral line, argon-ion laser

    Excitation Wavelength: 488 nm

    Emission Wavelength: 535 nm

  • 标记(Labeling)

    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.

  • 蛋白标记度(Protein Ratio)

    The FITC to protein molar ratio is 1.5-3.5.

  • 纯度(Purity)

    >90% as determined by SDS-PAGE.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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  • 电泳(SDS-PAGE)

    GUCY2C SDS-PAGE

    FITC-Labeled Human GUCY2C, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • 活性(Bioactivity)-ELISA

     GUCY2C ELISA

    Immobilized Monoclonal Anti-Human GUCY2C Antibody, Human IgG1 at 1 μg/mL (100 μL/well) can bind FITC-Labeled Human GUCY2C, Fc Tag (Cat. No. GUC-HF255) with a linear range of 0.01-0.313 μg/mL (QC tested).

    Protocol

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背景介绍

GUCY2C (Guanylyl Cyclase C), also known as heat-stable enterotoxin receptor, is a type I transmembrane protein of the guanylate cyclase (gc) family that signal by producing cGMP. Guanylate cyclase C (GUCY2C) and its hormones guanylin and uroguanylin have recently emerged as one paracrine axis defending intestinal mucosal integrity against mutational, chemical, and inflammatory injury. GUCY2C murine CAR-T cells recognized and killed human colorectal cancer cells endogenously expressing GUCY2C. Thus, we have identified a human GUCY2C-specific CAR-T cell therapy approach that may be developed for the treatment of GUCY2C-expressing metastatic colorectal cancer.

前沿进展

 
药物研发进展
  • 英文全称:

    Heat-stable enterotoxin receptor

  • 中文全称:

    热稳定肠毒素受体

  • 种类:

  • 上市药物数量:

    1 详情

  • 临床药物数量:

    5 详情

  • 最高研发阶段:

    批准上市

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