Biotinylated Human Growth Hormone R Protein, Fc,Avitag™ (MALS verified)

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GHR-H82F3-25ug
¥3675.00
GHR-H82F3-200ug
¥14490.00
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产品详情

  • 分子别名(Synonym)

    GHR,GHBP,GH receptor

  • 表达区间及表达系统(Source)

    Biotinylated Human Growth Hormone R Protein, Fc,Avitag (GHR-H82F3) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Tyr 264 (Accession # P10912-1).

    Predicted N-terminus: Ala 27

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    Growth Hormone R Structure

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 55 kDa. The protein migrates as 65-90 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 标记(Labeling)

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • 蛋白标记度(Protein Ratio)

    Passed as determined by the HABA assay / binding ELISA.

  • 纯度(Purity)

    >90% as determined by SDS-PAGE.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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数据展示

  • 电泳(SDS-PAGE)

    Growth Hormone R SDS-PAGE

    Biotinylated Human Growth Hormone R Protein, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    Growth Hormone R SEC-MALS

    The purity of Biotinylated Human Growth Hormone R Protein, Fc,Avitag (Cat. No. GHR-H82F3) is more than 85% and the molecular weight of this protein is around 130-150 kDa verified by SEC-MALS.

    Report
  • 活性(Bioactivity)-ELISA

     Growth Hormone R ELISA

    Immobilized Human GH, premium grade (Cat. No. GH1-H4118) at 1 μg/mL (100 μL/well) can bind Biotinylated Human Growth Hormone R Protein, Fc,Avitag (Cat. No. GHR-H82F3) with a linear range of 0.2-8 ng/mL (QC tested).

    Protocol

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背景介绍

Growth hormone receptor (GHR) is also known as somatotropin receptor, growth hormone-binding protein (GHBR), which belongs to the type I cytokine receptor family or Type 1 subfamily. GHR contains one fibronectin type-III domain. GHR / GHBR is expressed in various tissues with high expression in liver and skeletal muscle. The soluble form (GHBP) is produced by phorbol ester-promoted proteolytic cleavage at the cell surface (shedding) by ADAM17/TACE. GHR is receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway. The soluble form (GHBP) acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling.

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药物研发进展
  • 英文全称:

    Growth hormone receptor

  • 中文全称:

    生长激素受体

  • 种类:

  • 上市药物数量:

    28 详情

  • 临床药物数量:

    32 详情

  • 最高研发阶段:

    批准上市

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