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分子别名（Synonym）
GPRC5D, G-protein coupled receptor family C group 5 member D
表达区间及表达系统（Source）
Biotinylated Human GPRC5D Full Length Protein (VLP) (GPD-H82P3) is expressed from human 293 cells (HEK293). It contains AA Tyr 2 - Val 345 (Accession # Q9NZD1-1).
Predicted N-terminus: Asp
蛋白结构（Molecular Characterization）
Virus-like particles(VLPs) are formed by self-assembly of capsid proteins from viruses. Membrane Proteins can be constituted in-situ with VLPs produced from HEK293 cell cultures. These VLPs concentrate conformationally intact membrane proteins directly on the cell surface and produce soluble, high-concentration proteins perfect for immunization and antibody screening.
The VLPs provide the display of properly folded membrane proteins in their native cellular membrane in a compact size of 100~300 nm diameter (similar to the size of most viruses) making it optimal targets for dendritic cells in vivo and surface attachment for phage display.
标记（Labeling）
The VLP is biotinylated with propriety technology.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
纯度（Purity）
>95% as determined by SEC-HPLC.
制剂（Formulation）
The VLPs are highly immunogenic, so the immunization strategy should be optimized (antigen dose, regimen and adjuvant).
Supplied as 0.2 μm filtered solution in PBS, Arginine, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
运输（Shipping）
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储（Storage）
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 12 months under sterile conditions.
质量管理控制体系（QMS）
- *The isotype control of empty/mock VLP (Cat. No. VLP-N82P3) is sold separately and not included in protein, you can follow the link for product information.
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数据展示
SEC-HPLC
The purity of Biotinylated Human GPRC5D Full Length Protein (VLP) (Cat. No. GPD-H82P3) was greater than 95% as determined by SEC-HPLC.
活性（Bioactivity）-ELISA
Immobilized Biotinylated Human GPRC5D Full Length Protein (VLP) (Cat. No. GPD-H82P3) at 2 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-GPRC5D Antibody, Human IgG4 with a linear range of 0.1-3 ng/mL (QC tested).
Protocol
ELISA comparison of biotinylated full-length human GPRC5D (virus-like particles) using anti-GPRC5D antibody shows ACRO (Cat. No. GPD-H82P3) has superior bioactivity to Company S (Routinely tested).
Protocol
均一性（Identity）-DLS
The mean peak Radius of VLP is 60-80 nm with more than 95% intensity as determined by dynamic light scattering (DLS).
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