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分子别名（Synonym）
Glypican 1, GPC1
表达区间及表达系统（Source）
Cynomolgus Glypican 1 Protein, His Tag (GP1-C52H3) is expressed from human 293 cells (HEK293). It contains AA Asp 24 - Ser 530 (Accession # XP_005574876.1).
Predicted N-terminus: Asp 24
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 57.8 kDa. The protein migrates as 60-95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
Cynomolgus Glypican 1 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-ELISA
Immobilized Human FGF basic, premium grade (Cat. No. BFF-H4117) at 1 μg/mL (100 μL/well) can bind Cynomolgus Glypican 1 Protein, His Tag (Cat. No. GP1-C52H3) with a linear range of 0.004-0.125 μg/mL (QC tested).
Protocol
Immobilized Cynomolgus Glypican 1 Protein, His Tag (Cat. No. GP1-C52H3) at 1 μg/mL (100 μL/well) can bind Biotinylated Human FGF basic, Avitag,His Tag (Cat. No. FGC-H81E3) with a linear range of 0.3-20 ng/mL (Routinely tested).
Protocol
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