产品详情
分子别名(Synonym)
Glycoprotein B/gB (HSV-2)
表达区间及表达系统(Source)
Biotinylated HSV-2 (strain 333) Glycoprotein B (gB), His,Avitag (GLB-V82E3) is expressed from human 293 cells (HEK293). It contains AA Glu 98 - Ala 730 (Accession # P06763).
Predicted N-terminus: Glu 98
Request for sequence蛋白结构(Molecular Characterization)

Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 76.0 kDa. The protein migrates as 90-110 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a trimer.
标记(Labeling)
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度(Protein Ratio)
Passed as determined by the HABA assay / binding ELISA.
纯度(Purity)
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂(Formulation)
Supplied as 0.2 μm filtered solution in PBS, 0.5 M Arginine, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
运输(Shipping)
This product is supplied and shipped with dry ice, please inquire the shipping cost.
存储(Storage)
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
质量管理控制体系(QMS)
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数据展示
电泳(SDS-PAGE)

Biotinylated HSV-2 (strain 333) Glycoprotein B (gB), His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS

The purity of Biotinylated HSV-2 (strain 333) Glycoprotein B (gB), His,Avitag (Cat. No. GLB-V82E3) is more than 90% and the molecular weight of this protein is around 235-270 kDa verified by SEC-MALS.
Report
活性(Bioactivity)-ELISA

Immobilized Biotinylated HSV-2 (strain 333) Glycoprotein B (gB), His,Avitag (Cat. No. GLB-V82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Mouse anti-Herpes virus 1 and 2, clone T111, Mouse IgG1 with a linear range of 0.02-1 ng/mL (QC tested).
Protocol
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