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Ebolavirus (subtype Bundibugyo, strain Uganda 2007) Envelope Glycoprotein 1 (GP1) Protein, His Tag

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BEP-V5221-100ug
¥2730.00
BEP-V5221-1mg (500ug X 2)
¥15750.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    Glycoprotein 1/GP1 (EBOV)

  • 表达区间及表达系统(Source)

    Ebolavirus (subtype Bundibugyo, strain Uganda 2007) GP1 Protein, His Tag (BEP-V5221) is expressed from human 293 cells (HEK293). It contains AA Ile 33 - Gln 304 (Accession # B8XCN0).

    Predicted N-terminus: Ile 33

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    Glycoprotein 1/GP1 (EBOV) Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 31.8 kDa. The protein migrates as 40-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 内毒素(Endotoxin)

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • 纯度(Purity)

    >90% as determined by SDS-PAGE.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

数据展示

  • 电泳(SDS-PAGE)

    Glycoprotein 1/GP1 (EBOV) SDS-PAGE

    Ebolavirus (subtype Bundibugyo, strain Uganda 2007) GP1 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

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背景介绍

EBOV encodes seven structural proteins: nucleoprotein (NP), polymerase cofactor (VP35), (VP40), GP, transcription activator (VP30), VP24, and RNA polymerase (L). GP protein contains 160-kDa envelope-attached glycoprotein (GP) and a 110 kDa secreted glycoprotein (sGP). GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . GP1 is responsible for binding to the receptor(s) on target cells. Interacts with CD209/DC-SIGN and CLEC4M/DC-SIGNR which act as cofactors for virus entry into the host cell. GP2 acts as a class I viral fusion protein. GP1,2 mediates endothelial cell activation and decreases endothelial barrier function. sGP seems to possess an anti-inflammatory activity as it can reverse the barrier-decreasing effects of TNF alpha.

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