产品详情
分子别名(Synonym)
TNFSF18, AITRL, TL6, hGITRL, GITR Ligand
表达区间及表达系统(Source)
Human GITR Ligand Protein, Fc Tag, active trimer (GIL-H5263) is expressed from human 293 cells (HEK293). It contains AA Gln 50 - Ser 177 (Accession # AAH69319.1).
Predicted N-terminus: Pro
蛋白结构(Molecular Characterization)
This protein carries a human IgG1 Fc tag at the N-terminus.
The protein has a calculated MW of 71.2 kDa. The protein migrates as 80-95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method / rFC method.
纯度(Purity)
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系(QMS)
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数据展示
电泳(SDS-PAGE)

Human GITR Ligand Protein, Fc Tag, active trimer on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS

The purity of Human GITR Ligand Protein, Fc Tag, active trimer (Cat. No. GIL-H5263) is more than 90% and the molecular weight of this protein is around 145-180 kDa verified by SEC-MALS.
Report
活性(Bioactivity)-ELISA

Immobilized Human GITR Ligand Protein, Fc Tag, active trimer (Cat. No. GIL-H5263) at 10 μg/mL (100 μL/well) can bind Human GITR Protein, Mouse IgG2a Fc Tag with a linear range of 0.08-2 ng/mL (QC tested).
Protocol
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