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分子别名（Synonym）
CD224, D22S672, D22S732, GGT, GGT 1, GGTD, GTG
表达区间及表达系统（Source）
Human GGT1 Protein, His Tag (GG1-H5243) is expressed from human 293 cells (HEK293). It contains AA Pro 27 - Tyr 569 (Accession # P19440).
Predicted N-terminus: His
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 60.5 kDa. The protein migrates as 23-25 kDa and 50-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method / rFC method.
纯度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
Human GGT1 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human GGT1 Protein, His Tag (Cat. No. GG1-H5243) is more than 90% and the molecular weight of this protein is around 55-80 kDa verified by SEC-MALS.
Report
活性（Bioactivity）
Measured by its ability to hydrolyze glutathione to Glu and Cys-Gly. The specific activity is >1300 pmol/min/μg (QC tested).
Protocol
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