Human GDNF / ATF / hGDNF Protein (E. coli), Research Grade
academia only

  • This product is specifically for academia customers.
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价格
Qty.
GDF-H5119-20ug
¥1600.00
GDF-H5119-50ug
¥4250.00
GDF-H5119-1mg (200ug X 5)
¥27600.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    GDNF, ATF

  • 表达区间及表达系统(Source)

    Human GDNF Protein (E. coli), Research Grade (GDF-H5119) is expressed from E. coli cells. It contains AA Ser 78 - Ile 211 (Accession # P39905-1).

    Predicted N-terminus: Met

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    GDNF Structure

    This protein carries no "tag".

    The protein has a calculated MW of 15.2 kDa. The protein migrates as 15 kDa±3 kDa under reducing (R) condition, and 30 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE).

  • 内毒素(Endotoxin)

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • 纯度(Purity)

    >95% as determined by SDS-PAGE.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in 20 mM Sodium Citrate, 150 mM NaCl, pH4.0 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 24 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程
  • 质量说明(Quality Description)

    ACRO's Research-grade products are suitable for a wide range of cell culture applications, particularly for research use in academic institutions. These products are sterilized by filtration before lyophilization. Typical specifications include endotoxin levels of <0.01 EU/μg and purities >95%. Biological activity is calibrated against WHO/NIBSC standards when available.
    ACRO's Premium-grade (Pre-GMP) products are characterized by their high quality and enhanced safety profiles, making them ideal for early-stage discovery and manufacturing processes in cell therapy companies. A key advantage is their seamless transition to corresponding GMP-grade versions. Biological activity is calibrated against WHO/NIBSC standards when available. Typical specifications include endotoxin levels of <0.01 EU/μg and purities >95%. In addition, rigorous testing is conducted to ensure the absence of mycoplasma, HCD, and HCP, thereby guaranteeing product safety.

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数据展示

  • 电泳(SDS-PAGE)

    GDNF SDS-PAGE

    Human GDNF Protein (E. coli), Research Grade on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • 活性(Bioactivity)-ELISA

     GDNF ELISA

    Immobilized Human GDNF Protein (E. coli), Research Grade (Cat. No. GDF-H5119) at 5 μg/mL (100 μL/well) can bind Human GFR alpha-1, His Tag (Cat. No. GF1-H5224) with a linear range of 0.2-13 ng/mL (QC tested).

    Protocol

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背景介绍

Glial cell line-derived neurotrophic factor (GDNF) is a neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. GDNF is a glycosylated, disulfide-bonded homodimer that is a distantly related member of the transforming growth factor-beta superfamily. The GDNF family members (consists of GDNF, neurturin, artemin and persephin) are structurally similar to transforming growth factor-beta. Unlike other members of the TGF-beta superfamily, which signal through the receptor serine-threonine kinases, GDNF family ligands activate intracellular signalling cascades via the receptor tyrosine kinase Ret. GDNF family ligands binds to GDNF family receptor alpha (GFRalpha) and then the GDNF family ligand-GFR alpha complex binds to and stimulates autophosphorylation of Ret.

前沿进展

 
药物研发进展
  • 英文全称:

    Glial cell line-derived neurotrophic factors

  • 中文全称:

    神经胶质细胞系衍生神经营养因子类

  • 种类:

  • 上市药物数量:

    0 详情

  • 临床药物数量:

    4 详情

  • 最高研发阶段:

    申请上市

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