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分子别名（Synonym）
GDF-8, Myostatin, GDF8, MSTN, Growth, differentiation factor 8
表达区间及表达系统（Source）
Biotinylated Human latent GDF-8 Protein, His Tag, primary amine labeling (GD8-H8243) is expressed from human 293 cells (HEK293). It contains AA Asn 24 - Ser 375 (Accession # O14793). It is the biotinylated form of Human latent GDF-8, His Tag (Cat. No. GD8-H5243).
Predicted N-terminus: His
Request for sequence蛋白结构（Molecular Characterization）
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 42.0 kDa. The protein migrates as 38 kDa and 50-53 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a dimer.
标记（Labeling）
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
纯度（Purity）
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in 0.2 M Arginine, PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
Biotinylated Human latent GDF-8 Protein, His Tag, primary amine labeling on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Human latent GDF-8 Protein, His Tag, primary amine labeling (Cat. No. GD8-H8243) is more than 95% and the molecular weight of this protein is around 90-110 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-SPR
Human Follistatin Protein, His Tag, premium grade (Cat. No. FON-H52H4) immobilized on CM5 Chip can bind Biotinylated Human latent GDF-8 Protein, His Tag (Cat. No. GD8-H8243) with an affinity constant of 248 nM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
Apitegromab captured on Protein A Chip can bind Biotinylated Human latent GDF-8 Protein, His Tag, primary amine labeling (Cat. No. GD8-H8243) with an affinity constant of 17.1 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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