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分子别名（Synonym）
GDF-8, Myostatin, GDF8, MSTN, Growth, differentiation factor 8
表达区间及表达系统（Source）
Biotinylated Cynomolgus latent GDF-8 Protein, Avitag,His Tag (GD8-C82Q3) is expressed from human 293 cells (HEK293). It contains AA Gly 19 - Ser 375 (Accession # Q95J86).
Predicted N-terminus: Gly
Request for sequence蛋白结构（Molecular Characterization）
This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 44.1 kDa. The protein migrates as 11-12 kDa, 40-43 kDa and 55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method / rFC method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
Biotinylated Cynomolgus latent GDF-8 Protein, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-ELISA
Immobilized Biotinylated Cynomolgus latent GDF-8 Protein, Avitag,His Tag (Cat. No. GD8-C82Q3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Apitegromab with a linear range of 0.06-1 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Cynomolgus latent GDF-8 Protein, Avitag,His Tag (Cat. No. GD8-C82Q3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human Activin RIIB Protein, Fc Tag (Cat. No. ACB-H5254) with a linear range of 0.002-0.313 μg/mL (Routinely tested).
Protocol
Immobilized Human Follistatin Protein, His Tag, premium grade (Cat. No. FON-H52H4) at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus latent GDF-8 Protein, Avitag,His Tag (Cat. No. GD8-C82Q3) with a linear range of 0.5-16 ng/mL (Routinely tested).
Protocol
Immobilized Human Activin RIIB Protein, Fc Tag (Cat. No. ACB-H5254) at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus latent GDF-8 Protein, Avitag,His Tag (Cat. No. GD8-C82Q3) with a linear range of 0.01-0.313 μg/mL (Routinely tested).
Protocol
活性（Bioactivity）-SPR
Apitegromab captured on Protein A Chip can bind Biotinylated Cynomolgus latent GDF-8 Protein, Avitag,His Tag (Cat. No. GD8-C82Q3) with an affinity constant of 25.1 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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