产品详情
分子别名(Synonym)
GDF15,GDF-15,MIC-1,MIC1,NAG-1,PDF,PLAB,PTGFB,NRG-1
表达区间及表达系统(Source)
Biotinylated Human GDF-15, Avitag, Fc Tag (GD5-H82F9) is expressed from human 293 cells (HEK293). It contains AA Ala 197 - Ile 308 (Accession # Q99988-1).
Predicted N-terminus: Gly
Request for sequence蛋白结构(Molecular Characterization)

This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a human IgG1 Fc tag.
The protein has a calculated MW of 40.4 kDa. The protein migrates as 50-55 kDa under reducing (R) condition, and 80-90 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
标记(Labeling)
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度(Protein Ratio)
Passed as determined by the HABA assay / binding ELISA.
纯度(Purity)
>90% as determined by SDS-PAGE.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系(QMS)
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数据展示
电泳(SDS-PAGE)

Biotinylated Human GDF-15, Avitag, Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS

The purity of Biotinylated Human GDF-15, Avitag, Fc Tag (Cat. No. GD5-H82F9) is more than 85% and the molecular weight of this protein is around 80-95 kDa verified by SEC-MALS.
Report
活性(Bioactivity)-ELISA

Immobilized Human GFR alpha-like, His Tag (Cat. No. GFA-H52H3) at 5 μg/mL (100 μL/well) can bind Biotinylated Human GDF-15, Avitag, Fc Tag (Cat. No. GD5-H82F9) with a linear range of 0.2-8 ng/mL (QC tested).
Protocol
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