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Human Flt-3 Ligand Protein, Research Grade
academia only

COADS MS
DS
  • This product is specifically for academia customers.
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货号-规格
价格
Qty.
FLL-H5219-20ug
¥1410.00
FLL-H5219-50ug
¥2570.00
FLL-H5219-1mg (200ug X 5)
¥16600.00
合计0件 产品金额¥ 0
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产品详情

  • 分子别名（Synonym）

    FLT3LG, FL, FLT3L, Flt3 ligand

  • 表达区间及表达系统（Source）

    Human Flt-3 Ligand Protein, Research Grade (FLL-H5219) is expressed from human 293 cells (HEK293). It contains AA Thr 27 - Pro 185 (Accession # P49771-1).

    Predicted N-terminus: Thr 27

    Request for sequence

  • 蛋白结构（Molecular Characterization）

    Flt-3 Ligand Structure

    This protein carries no "tag".

    The protein has a calculated MW of 18.0 kDa. The protein migrates as 24 kDa, 27 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 内毒素（Endotoxin）

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • 纯度（Purity）

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • 制剂（Formulation）

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法（Reconstitution）

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储（Storage）

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.

  • 质量管理控制体系（QMS）

    1. 质量管理体系（ISO, GMP）
    2. 质量优势
    3. 质控流程

  • 质量说明（Quality Description）

    ACRO's Research-grade products are suitable for a wide range of cell culture applications, particularly for research use in academic institutions. These products are sterilized by filtration before lyophilization. Typical specifications include endotoxin levels of <0.01 EU/μg and purities >95%. Biological activity is calibrated against WHO/NIBSC standards when available.
    ACRO's Premium-grade (Pre-GMP) products are characterized by their high quality and enhanced safety profiles, making them ideal for early-stage discovery and manufacturing processes in cell therapy companies. A key advantage is their seamless transition to corresponding GMP-grade versions. Biological activity is calibrated against WHO/NIBSC standards when available. Typical specifications include endotoxin levels of <0.01 EU/μg and purities >95%. In addition, rigorous testing is conducted to ensure the absence of mycoplasma, HCD, and HCP, thereby guaranteeing product safety.

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数据展示

  • 电泳（SDS-PAGE）

    Flt-3 Ligand SDS-PAGE

    Human Flt-3 Ligand Protein, Research Grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    Flt-3 Ligand SEC-MALS

    The purity of Human Flt-3 Ligand Protein, Research Grade (Cat. No. FLL-H5219) is more than 95% and the molecular weight of this protein is around 42-52 kDa verified by SEC-MALS.

    Report

  • 活性（Bioactivity）-ELISA

    Flt-3 Ligand ELISA

    Immobilized Human Flt-3 Ligand Protein, Research Grade (Cat. No. FLL-H5219) at 1 μg/mL (100 μL/well) can bind Human Flt-3, Fc Tag (Cat. No. FL3-H5258) with a linear range of 0.1-3.9 ng/mL (QC tested).

    Protocol

  • 活性（Bioactivity）-CELL BASE

    Flt-3 Ligand CELL

    Human Flt-3 Ligand Protein, Research Grade (Cat. No. FLL-H5219) stimulates proliferation of OCI-AML5 cells. The specific activity of Human Flt-3 Ligand Protein, Research Grade is > 5.00 x 10^5 IU/mg, which is calibrated against WHO Reference Reagent FMS-LIKE TYROSINE KINASE 3 LIGAND (Human, rDNA derived) NIBSC code: 96/532 (QC tested ).

    Protocol

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背景介绍

FMS-like tyrosine kinase 3 ligand (Flt-3 Ligand) is also known as FL, Flt3L and FLT3LG, is an α-helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages. FLT3LG is expressed as a noncovalentlylinked dimer by T cells and bone marrow and thymic fibroblasts. Each 36 kDa chain carries approximately 12 kDa of N- and O- linked carbohydrates. FLT3LG is structurally homologous to stem cell factor (SCF) and colony stimulating facor 1 (CSF-1). FLT3LG acts as a growth factor that increases the number of immune cells by activating the hematopoietic progenitors. It also induces the mobilization of the hematopoietic progenitors and stem cells in vivo which may help the system to kill cancer cells. FLT3LG induces the expansion of monocytes and immature dendritic cells as well as early B cell lineage differentiation. FLT3LG cooperates with IL2, IL6, IL7, and IL15 to induce NK cell development and with IL3, IL7 and IL11 to induce terminal B cell maturation. Animal studies also show FLT3LG to reduce the severity of experimentally induced allergic inflammation. FLT3LG is crucial for steady-state pDC and cDC development. A lack of FLT3L results in low levels of DCs.

前沿进展

 
药物研发进展
  • 英文全称：

    Fms-related tyrosine kinase 3 ligand

  • 中文全称：

    Flt3配体

  • 种类：

  • 上市药物数量：

    0 详情

  • 临床药物数量：

    1 详情

  • 最高研发阶段：

    临床二期

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