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Recombinant Fibronectin fragment, Research Grade
academia only

COADS MS
DS
  • This product is specifically for academia customers.
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FIN-H5117-500ug
¥640.00
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产品详情

  • 分子别名（Synonym）

    Fibronectin, FN1, CIG, ED-B, FINC, FN, FNZ, GFND, GFND2, LETS, MSF

  • 表达区间及表达系统（Source）

    Recombinant Fibronectin fragment, Research Grade (FIN-H5117) is expressed from E. coli cells. It contains AA Pro 1361 - Ser 1637 & Ala 1812 - Thr 2107 (Accession # P02751-15).

    Predicted N-terminus: Met

    Request for sequence

  • 蛋白结构（Molecular Characterization）

    Fibronectin Structure

    This protein carries no "tag".

    The protein has a calculated MW of 62.6 kDa. The protein migrates as 60 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

  • 内毒素（Endotoxin）

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • 纯度（Purity）

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-HPLC.

  • 制剂（Formulation）

    Supplied as 0.2 μm filtered solution in 12.5 mM Citric acid, pH6.2 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • 运输（Shipping）

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • 存储（Storage）

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C for 12 months under sterile conditions.

  • 质量管理控制体系（QMS）

    1. 质量管理体系（ISO, GMP）
    2. 质量优势
    3. 质控流程

  • 质量说明（Quality Description）

    ACRO's Research-grade products are suitable for a wide range of cell culture applications, particularly for research use in academic institutions. These products are sterilized by filtration before lyophilization. Typical specifications include endotoxin levels of <0.01 EU/μg and purities >95%. Biological activity is calibrated against WHO/NIBSC standards when available.
    ACRO's Premium-grade (Pre-GMP) products are characterized by their high quality and enhanced safety profiles, making them ideal for early-stage discovery and manufacturing processes in cell therapy companies. A key advantage is their seamless transition to corresponding GMP-grade versions. Biological activity is calibrated against WHO/NIBSC standards when available. Typical specifications include endotoxin levels of <0.01 EU/μg and purities >95%. In addition, rigorous testing is conducted to ensure the absence of mycoplasma, HCD, and HCP, thereby guaranteeing product safety.

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数据展示

  • 电泳（SDS-PAGE）

    Fibronectin SDS-PAGE

    Recombinant Fibronectin fragment, Research Grade on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-HPLC

    Fibronectin SEC-HPLC

    The purity of Recombinant Fibronectin fragment, Research Grade (Cat. No. FIN-H5117) was greater than 90% as determined by SEC-HPLC.

  • 活性（Bioactivity）-SPR

    Fibronectin SPR

    Recombinant Fibronectin fragment, Research Grade (Cat. No. FIN-H5117) immobilized on CM5 Chip can bind Heparin Sulfate with an affinity constant of 0.24 μM as determined in a SPR assay (Biacore T200) (QC tested).

    Protocol

  • Fibronectin SPR

    Biotinylated Human ITGA5&ITGB1 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT1-H82Wa) immobilized on SA Chip can bind Recombinant Fibronectin fragment, Research Grade (Cat. No. FIN-H5117) with an affinity constant of 43.4 nM as determined in a SPR assay (Biacore 8K) (QC tested).

    Protocol

  • Fibronectin SPR

    Biotinylated Human ITGA4&ITGB1 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT1-H82W1) immobilized on SA Chip can bind Recombinant Fibronectin fragment, Research Grade (Cat. No. FIN-H5117) with an affinity constant of 0.823 μM as determined in a SPR assay (Biacore 8K) (QC tested).

    Protocol

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背景介绍

Fibronectin (Fn) is a glycoprotein whose size ranges from 230 to 270 kDa and usually exists as a dimer, covalently linked by a pair of disulfide bonds at the C-termini. Each monomer consists of three repeating units: 12 Type I, 2 Type II, and 15–17 Type III domains which combined account for 90% of the FN sequence. The extracellular matrix (ECM) plays a key role as both structural scaffold and regulator of cell signal transduction in tissues. Fibronectin is one of the major ECM proteins in the trabecular meshwork (TM). It is found in the sheath material surrounding the elastin tendons that enter the TM from the ciliary muscle within the ciliary body. In times of ECM assembly and turnover, cells upregulate assembly of the ECM protein, FN. FN is assembled by cells into viscoelastic fibrils that can bind upward of 40 distinct growth factors and cytokines. These fibrils play a key role in assembling a provisional ECM during embryonic development and wound healing. Fibril assembly is also often upregulated during disease states, including cancer and fibrotic diseases.

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