关注公众号
收藏产品 产品详情
分子别名（Synonym）
FGF2, BFGF, FGFB, FGF basic, HBGF-2
产品描述（Product Details）
Our Recombinant Human FGF basic Superior Stable Mutant Protein, developed via an AI-modified and experimentally validated closed-loop efficient protein design platform, is the optimal FGF2 mutant with superior thermal stability and activity compared to wild-type FGF2, making it more suitable for various stem cell culture processes. This reduces medium replacement frequency, lowers contamination risks from frequent operations, and saves costs/time for stem cell culture by minimizing reagent consumption and labor, especially in large-scale applications.
It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.
蛋白结构（Molecular Characterization）
This protein carries no "tag".
The protein has a calculated MW of 16.5 kDa. The protein migrates as 17 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
内毒素（Endotoxin）
Less than 0.01 EU per μg by the LAL method / rFC method.
宿主蛋白残留（Host Cell Protein）
<0.5 ng/µg of protein tested by ELISA.
宿主核酸残留（Host Cell DNA）
<0.02 ng/μg of protein tested by qPCR.
无菌（Sterility）
Negative
支原体（Mycoplasma）
Negative
纯度（Purity）
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
质量说明（Quality Description）
ACRO's Research-grade products are suitable for a wide range of cell culture applications, particularly for research use in academic institutions. These products are sterilized by filtration before lyophilization. Typical specifications include endotoxin levels of <0.01 EU/μg and purities >95%. Biological activity is calibrated against WHO/NIBSC standards when available.
ACRO's Premium-grade (Pre-GMP) products are characterized by their high quality and enhanced safety profiles, making them ideal for early-stage discovery and manufacturing processes in cell therapy companies. A key advantage is their seamless transition to corresponding GMP-grade versions. Biological activity is calibrated against WHO/NIBSC standards when available. Typical specifications include endotoxin levels of <0.01 EU/μg and purities >95%. In addition, rigorous testing is conducted to ensure the absence of mycoplasma, HCD, and HCP, thereby guaranteeing product safety.
推荐产品
数据展示
电泳（SDS-PAGE）
Human FGF basic Superior Stable Mutant Protein, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human FGF basic Superior Stable Mutant Protein, premium grade (Cat. No. BFF-H5113) is more than 95% and the molecular weight of this protein is around 13-21 kDa verified by SEC-MALS.
Report
热稳定性（Heat Stability）
Recombinant Human FGF basic Superior Stable Mutant Protein Induces Proliferation of NIH-3T3 Mouse Fibroblast Cells. (A) Human FGF basic Superior Stable Mutant Protein, premium grade (Cat. No. BFF-H5113) or (B) wild-type (WT) Human FGF basic Protein, premium grade (Cat. No. BFF-H4117), were either untreated or incubated in culture media at 37°C for 3 days. Following 3 days of treatment at 37°C, Recombinant Human FGF basic Superior Stable Mutant Protein retained activity comparable to the untreated heat-stable variant, demonstrating its enhanced thermal stability. In contrast, the WT Recombinant Human FGF basic Protein exhibited significant activity loss, indicating inferior thermal stability.
100 ng/mL Superior Stability for Recombinant Human FGF basic Superior Stable Mutant Protein (Cat. No. BFF-H5113) or (B) wild-type (WT) Human FGF basic Protein, premium grade (Cat. No. BFF-H4117), were incubated in culture media at 37°C for 0h, 8h, 24h, 48h and 72h. The concentration of residual FGF2 was detected by resDetect™ Human FGF2 ELISA Kit (Residue Testing) (Cat. No. RES-A009).
Following 72h of treatment at 37°C, Recombinant Human FGF basic Superior Stable Mutant Protein retained higher concentration comparable to the WT FGF2 protein, demonstrating its enhanced thermal stability. In contrast, the WT Recombinant Human FGF basic Protein exhibited significant concentration loss, indicating inferior thermal stability.
活性（Bioactivity）-CELL BASE
Human FGF basic Superior Stable Mutant Protein, premium grade (Cat. No. BFF-H5113) stimulates proliferation of NIH/3T3 cells. The specific activity of Human FGF basic Superior Stable Mutant Protein, premium grade is > 1.60 x 10^7 IU/mg, which is calibrated against Basic Fibroblast Growth Factor WHO International Standard (NIBSC code: 90/712) (QC tested).
Protocol
活性（Bioactivity）-Stem Cell Culture
100 ng/mL of either Recombinant Human FGF basic Superior Stable Mutant Protein (Cat. No. BFF-H5113, designated as HS-FGF2) or wild-type (WT) Human FGF basic Protein of premium grade (Cat. No. BFF-H4117, designated as WT-FGF2) was added to the iPSC culture system. The culture protocol involved daily medium replacement or a reduced feeding schedule with medium changes every 2 days. Cell counts were assessed via AO/PI staining from passage 1 to passage 3.
The results indicated that the Recombinant Human FGF basic Superior Stable Mutant Protein significantly promoted stem cell proliferation, maintaining high cell numbers even under both daily feeding and the reduced 2-day feeding regimen, with performance comparable to that of competitor T. In contrast, the WT Recombinant Human FGF basic Protein showed a marked decrease in cell numbers under the reduced 2-day feeding condition (Data is the average of 3 independent experiments, error bars ± SD).
100 ng/mL of either Recombinant Human FGF basic Superior Stable Mutant Protein (Cat. No. BFF-H5113, designated as HS-FGF2) or wild-type (WT) Human FGF basic Protein of premium grade (Cat. No. BFF-H4117, designated as WT-FGF2) was added to the iPSC culture system. The culture protocol involved daily medium replacement or a reduced feeding schedule with medium changes every 2 days. The expression of stem cell marker genes (OCT4 and SOX2) was detected by FACS and immunofluorescence in passage 3.
The results indicated that the Recombinant Human FGF basic Superior Stable Mutant Protein significantly maintained stem cell self-renewal, maintaining high expression of stem cell markers even under both daily feeding and the reduced 2-day feeding regimens, with performance comparable to that of competitor T. In contrast, the WT Recombinant Human FGF basic Protein showed a marked decrease in stem cell gene expression under the reduced 2-day feeding condition.
100 ng/mL of either Recombinant Human FGF basic Superior Stable Mutant Protein (Cat. No. BFF-H5113, designated as HS-FGF2) or wild-type (WT) Human FGF basic Protein of premium grade (Cat. No. BFF-H4117, designated as WT-FGF2) was added to the iPSC culture system. The culture protocol involved daily medium replacement or a reduced feeding schedule with medium changes every 2 days. The expression of stem cell marker genes (OCT4 and SOX2) was detected by FACS and immunofluorescence in passage 3.
The results indicated that the Recombinant Human FGF basic Superior Stable Mutant Protein significantly maintained stem cell self-renewal, maintaining high expression of stem cell markers even under both daily feeding and the reduced 2-day feeding regimens, with performance comparable to that of competitor T. In contrast, the WT Recombinant Human FGF basic Protein showed a marked decrease in stem cell gene expression under the reduced 2-day feeding condition.
活性（Bioactivity）-ELISA
Immobilized Human FGF basic Superior Stable Mutant Protein, premium grade (Cat. No. BFF-H5113) at 2 μg/mL (100 μL/well) can bind Human Glypican 3 Protein, Fc Tag (Cat. No. GP3-H5258) with a linear range of 0.2-6 ng/mL (QC tested).
Protocol
Immobilized Human FGF basic Superior Stable Mutant Protein, premium grade (Cat. No. BFF-H5113) at 2 μg/mL (100 μL/well) can bind Human Glypican 1, Fc Tag (Cat. No. GP1-H5254) with a linear range of 0.1-6 ng/mL (Routinely tested).
Protocol
用户评价 发表评论
药物研发进展 
联系我们 
项目合作 