产品详情
分子别名(Synonym)
FGF8, Fibroblast growth factor 8, FGF-8,Androgen-induced growth factor,Heparin-binding growth factor 8 , AIGF
表达区间及表达系统(Source)
Human FGF-8b Protein, Research Grade (FGB-H5116) is expressed from E. coli cells. It contains AA Gln 23 - Arg 215 (Accession # P55075-3).
Predicted N-terminus: Met
Request for sequence蛋白结构(Molecular Characterization)

This protein carries no "tag".
The protein has a calculated MW of 22.5 kDa. The protein migrates as 25 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
内毒素(Endotoxin)
Less than 0.01 EU per μg by the LAL method / rFC method.
纯度(Purity)
>95% as determined by SDS-PAGE.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系(QMS)
质量说明(Quality Description)
ACRO's Research-grade products are suitable for a wide range of cell culture applications, particularly for research use in academic institutions. These products are sterilized by filtration before lyophilization. Typical specifications include endotoxin levels of <0.01 EU/μg and purities >95%. Biological activity is calibrated against WHO/NIBSC standards when available.
ACRO's Premium-grade (Pre-GMP) products are characterized by their high quality and enhanced safety profiles, making them ideal for early-stage discovery and manufacturing processes in cell therapy companies. A key advantage is their seamless transition to corresponding GMP-grade versions. Biological activity is calibrated against WHO/NIBSC standards when available. Typical specifications include endotoxin levels of <0.01 EU/μg and purities >95%. In addition, rigorous testing is conducted to ensure the absence of mycoplasma, HCD, and HCP, thereby guaranteeing product safety.
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数据展示
电泳(SDS-PAGE)

Human FGF-8b Protein, Research Grade on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
活性(Bioactivity)-ELISA

Immobilized Human FGF-8b Protein, Research Grade (Cat. No. FGB-H5116) at 5 μg/mL (100 μL/well) can bind Human FGF R3 (IIIb), Fc Tag (Cat. No. FGB-H5259) with a linear range of 39-2500 ng/mL (QC tested).
Protocol
活性(Bioactivity)-CELL BASE

Human FGF-8b Protein, Research Grade (Cat. No. FGB-H5116) stimulates proliferation of NIH-3T3 cells. The specific activity of Human FGF-8b Protein, Research Grade is > 2.00 x 10^3 U/mg (QC tested).
Protocol
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