Human FGF-8b Protein, Research Grade
academia only

  • This product is specifically for academia customers.
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货号-规格
价格
Qty.
FGB-H5116-20ug
¥1150.00
FGB-H5116-100ug
¥4010.00
FGB-H5116-1mg (200ug X 5)
¥20620.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    FGF8, Fibroblast growth factor 8, FGF-8,Androgen-induced growth factor,Heparin-binding growth factor 8 , AIGF

  • 表达区间及表达系统(Source)

    Human FGF-8b Protein, Research Grade (FGB-H5116) is expressed from E. coli cells. It contains AA Gln 23 - Arg 215 (Accession # P55075-3).

    Predicted N-terminus: Met

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    FGF-8b Structure

    This protein carries no "tag".

    The protein has a calculated MW of 22.5 kDa. The protein migrates as 25 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

  • 内毒素(Endotoxin)

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • 纯度(Purity)

    >95% as determined by SDS-PAGE.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 24 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程
  • 质量说明(Quality Description)

    ACRO's Research-grade products are suitable for a wide range of cell culture applications, particularly for research use in academic institutions. These products are sterilized by filtration before lyophilization. Typical specifications include endotoxin levels of <0.01 EU/μg and purities >95%. Biological activity is calibrated against WHO/NIBSC standards when available.
    ACRO's Premium-grade (Pre-GMP) products are characterized by their high quality and enhanced safety profiles, making them ideal for early-stage discovery and manufacturing processes in cell therapy companies. A key advantage is their seamless transition to corresponding GMP-grade versions. Biological activity is calibrated against WHO/NIBSC standards when available. Typical specifications include endotoxin levels of <0.01 EU/μg and purities >95%. In addition, rigorous testing is conducted to ensure the absence of mycoplasma, HCD, and HCP, thereby guaranteeing product safety.

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数据展示

  • 电泳(SDS-PAGE)

    FGF-8b SDS-PAGE

    Human FGF-8b Protein, Research Grade on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • 活性(Bioactivity)-ELISA

     FGF-8b ELISA

    Immobilized Human FGF-8b Protein, Research Grade (Cat. No. FGB-H5116) at 5 μg/mL (100 μL/well) can bind Human FGF R3 (IIIb), Fc Tag (Cat. No. FGB-H5259) with a linear range of 39-2500 ng/mL (QC tested).

    Protocol
  • 活性(Bioactivity)-CELL BASE

     FGF-8b CELL

    Human FGF-8b Protein, Research Grade (Cat. No. FGB-H5116) stimulates proliferation of NIH-3T3 cells. The specific activity of Human FGF-8b Protein, Research Grade is > 2.00 x 10^3 U/mg (QC tested).

    Protocol

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背景介绍

FGF-8 is a member of the fibroblast growth factor family that was originally discovered as a growth factor essential for the androgen-dependent growth of mouse mammary carcinoma cells (1-3). Alternate splicing of mouse FGF-8 mR generates eight secreted isoforms, designated a-h, but only FGF-8a, b, e and f exist in humans (4). FGF-8 contains a 22 amino acid (aa) signal sequence, an N‑terminal domain that varies according to the isoform (30 aa for FGF-8b; 20 aa for the shortest, FGF-8a), a 125 aa FGF domain and a 37 aa proline‑rich C‑terminal sequence. The FGF domain of FGF-8 shares the most aa identity with FGF17 (75%) and FGF-18 (67%), and the three form an FGF subfamily (2). Mouse FGF-8b shares 100% aa identity with human FGF-8b. FGF-8 is widely expressed during embryogenesis, and mediates epithelial-mesenchymal transitions. It plays an organizing and inducing role during gastrulation, and regulates patterning of the midbrain/hindbrain, eye, ear, limbs and heart in the embryo (2, 5 - 8). The isoforms may play different roles in development. FGF-8b shows the strongest receptor affinity and oncogenic transforming capacity although FGF-8a and FGF-8e are also transforming and have been found in human prostate, breast or ovarian tumors (1, 5, 9-12). FGF-8 shows limited expression in the normal adult, but low levels are found in the reproductive and genitourinary tract, peripheral leukocytes and bone marrow hematopoietic cells (3, 9, 13).

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