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分子别名（Synonym）
FGF-5, Fibroblast growth factor 5, HBGF-5
表达区间及表达系统（Source）
Human FGF-5 Protein, His Tag (FG5-H51H3) is expressed from E. coli cells. It contains AA Glu 23 - Gly 268 (K238N, P245S) (Accession # P12034-1).
Predicted N-terminus: Glu 23
Request for sequence蛋白结构（Molecular Characterization）
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 29.4 kDa. The protein migrates as 35-38 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
Human FGF-5 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-SPR
Human FGF-5 Protein, His Tag (Cat. No. FG5-H51H3) captured on CM5 chip via anti-His antibody can bind Human FGF R2 (IIIb), Fc Tag (Cat. No. FGB-H5256) with an affinity constant of 63.3 nM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
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