关注公众号
收藏产品 产品详情
分子别名（Synonym）
FGF-4, Fibroblast growth factor 4, HST, HST-1
表达区间及表达系统（Source）
Human FGF-4 Protein, His Tag (FG4-H51H3) is expressed from E. coli cells. It contains AA Ser 54 - Leu 206 (Accession # P08620-1).
Predicted N-terminus: Met
Request for sequence蛋白结构（Molecular Characterization）
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 19.8 kDa. The protein migrates as 23-24 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
内毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method / rFC method.
纯度（Purity）
>90% as determined by SDS-PAGE.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in MOPS, Na2SO4, Arginine with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
推荐产品
数据展示
电泳（SDS-PAGE）
Human FGF-4 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
活性（Bioactivity）-ELISA
Immobilized Human FGF-4 Protein, His Tag (Cat. No. FG4-H51H3) at 5 μg/mL (100 μL/well) can bind Human FGF R3 (IIIb), Fc Tag (Cat. No. FGB-H5259) with a linear range of 1-39 ng/mL (QC tested).
Protocol
Immobilized Human FGF-4 Protein, His Tag (Cat. No. FG4-H51H3) at 5 μg/mL (100 μL/well) can bind Human FGF R3 (IIIc), Fc Tag (Cat. No. FGC-H5256) with a linear range of 1-39 ng/mL (Routinely tested).
Protocol
活性（Bioactivity）-SPR
Human FGF R4, Fc Tag (Cat. No. FG4-H5253) immobilized on CM5 Chip can bind Human FGF-4 Protein, His Tag (Cat. No. FG4-H51H3) with an affinity constant of 1.48 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
用户评价 发表评论
药物研发进展 
联系我们 
项目合作 