Human Fetuin B Protein, His Tag

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Qty.
FEB-H52H7-1mg (500ug X 2)
¥15750.00
FEB-H52H7-100ug
¥2625.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    FETUB,Fetuin-B,16G2,Gugu

  • 表达区间及表达系统(Source)

    Human Fetuin B, His Tag (FEB-H52H7) is expressed from human 293 cells (HEK293). It contains AA Cys 16 - Pro 382 (Accession # AAH69670 ).

    Predicted N-terminus: Cys 16

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    Fetuin B Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 42.4 kDa. The protein migrates as 53-58 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 内毒素(Endotoxin)

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • 纯度(Purity)

    >95% as determined by SDS-PAGE.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH8.0 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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数据展示

  • 电泳(SDS-PAGE)

    Fetuin B SDS-PAGE

    Human Fetuin B, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

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背景介绍

Fetuin-B (FETUB), a second member of the fetuin family in mammals, is also known as Fetuin-like protein IRL685, 16G2 and Gugu, which is expressed in liver and testis and contains 2 cystatin fetuin-B-type domains. FETUB is a protease inhibitor required for egg fertilization. FETUB is also required to prevent premature zona pellucida hardening before fertilization, probably by inhibiting the protease activity of ASTL, a protease that mediates the cleavage of ZP2 and triggers zona pellucida hardening (By similarity). As a liver-derived plasma protein, FETUB is essential for fertilization.

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