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分子别名（Synonym）
FcRn, FCGRT & B2M
表达区间及表达系统（Source）
Biotinylated Canine FCGRT&B2M Heterodimer Protein, His,Avitag&Tag Free (FCM-C82W9) is expressed from human 293 cells (HEK293). It contains AA Ala 23 - Pro 298 & Val 28 - Asn 125 (Accession # XP_038512242.1 & NP_001271408.1).
Predicted N-terminus: Ala 23 | Val 28
Request for sequence蛋白结构（Molecular Characterization）
Biotinylated Canine FCGRT&B2M Heterodimer Protein, His,Avitag&Tag Free, produced by co-expression of FCGRT and B2M, has a calculated MW of 28.3 kDa (FCGRT) & 11.5 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) and subunit Beta-2 microglobulin (B2M) contains no tag at the C-terminus. The protein migrates as 38-40 kDa (FCGRT) and＜14.4 kDa (B2M) under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记（Labeling）
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度（Protein Ratio）
Passed as determined by the HABA assay / binding ELISA.
纯度（Purity）
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
制剂（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系（QMS）
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数据展示
电泳（SDS-PAGE）
Biotinylated Canine FCGRT&B2M Heterodimer Protein, His,Avitag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Biotinylated Canine FCGRT&B2M Heterodimer Protein, His,Avitag&Tag Free (Cat. No. FCM-C82W9) is more than 95% and the molecular weight of this protein is around 43-53 kDa verified by SEC-MALS.
Report
活性（Bioactivity）-ELISA
Immobilized Monoclonal Anti-Human CTLA-4 Antibody, Human IgG1 at 1 μg/mL (100 μL/well) can bind Biotinylated Canine FcRn / FCGRT&B2M Heterodimer, His,Avitag&Tag Free (Cat. No. FCM-C82W9) with a linear range of 0.039-2.5 μg/mL (Routinely tested).
Protocol
活性（Bioactivity）-SPR
Biotinylated Canine FCGRT&B2M Heterodimer, His,Avitag&Tag Free (Cat. No. FCM-C82W9) immobilized on SA Chip can bind Ipilimumab with an affinity constant of 42.5 nM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
Biotinylated Canine FCGRT&B2M Heterodimer, His,Avitag&Tag Free (Cat. No. FCM-C82W9) captured on CM5 chip via anti-His antibody can bind Ipilimumab with an affinity constant of 0.267 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Biotinylated Canine FCGRT&B2M Heterodimer, His,Avitag&Tag Free (Cat. No. FCM-C82W9) immobilized on SA Chip can bind Trastuzumab with an affinity constant of 44.6 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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