Human Fc Receptor Blocking Solution Protein, Tag Free

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货号-规格
价格
Qty.
FCN-H5213-50tests
¥750.00
FCN-H5213-200tests
¥1650.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    Fc Receptor Blocking Solution

  • 表达区间及表达系统(Source)

    Human Fc Receptor Blocking Solution Protein, Tag Free (FCN-H5213) is expressed from human 293 cells (HEK293).

  • 蛋白结构(Molecular Characterization)

    This protein carries no "tag".

    The protein has a calculated MW of 31.2 kDa.

  • 内毒素(Endotoxin)

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • 制剂(Formulation)

    Supplied as 0.2 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 运输(Shipping)

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • 存储(Storage)

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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数据展示

  • 活性(Bioactivity)-FACS

     Fc Receptor Blocking Solution FACS

    Flow cytometric analysis was performed on THP-1 cells that were either treated with Human Fc Receptor Blocking Solution Protein, Tag Free (Cat. No. FCN-H5213) using a 1:20 dilution (5 μL of antibody stock per 1e6 cells in a final volume of 100 µL) or left untreated. All cells were stained with an irrelevant PE-conjugated mouse IgG2a antibody. PE signal was used to evaluate the blocking effect (QC tested).

    Protocol

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背景介绍

Fc receptors expressed on the surface of immune cells such as monocytes, macrophages, dendritic cells, and certain lymphocyte subsets can bind to the Fc region of antibodies in a non‑antigen‑specific manner. This Fc–Fc receptor interaction often leads to high background signals in flow cytometry, immunofluorescence, and other immunoassays, complicating the interpretation of true antigen‑specific binding. To address this, Fc receptor blocking reagents are used prior to antibody staining to occupy Fc receptors, thereby preventing non‑specific binding and improving assay specificity and signal‑to‑noise ratio. The blocking efficiency may vary depending on the antibody isotype, Fc receptor subtype, and cell type tested.

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