PE-Labeled Human FAP Protein, His Tag (Site-specific conjugation)

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FAP-HP245-25tests
¥3700.00
FAP-HP245-200tests
¥21840.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    FAP,FAPalpha,SIMP,Seprase,APCE

  • 表达区间及表达系统(Source)

    PE-Labeled Human FAP, His Tag (FAP-HP245) is produced via site-specific conjugation of PE to Human FAP, His Tag under optimal conditions with a proprietary technology. Human FAP, His Tag is expressed from human 293 cells (HEK293). It contains AA Leu 26 - Asp 760 (Accession # Q12884-1).

    Predicted N-terminus: His

    Request for sequence

  • 蛋白结构(Molecular Characterization)

    FAP Structure

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 93.1 kDa.

  • 偶联(Conjugate)

    PE

    Excitation Wavelength: 488 nm / 561 nm

    Emission Wavelength: 575 nm

  • 应用说明(Application)

    Please note that this product is NOT compatible to streptavidin detection system.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, 0.5% BSA, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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数据展示

  • 活性(Bioactivity)-FACS

     FAP FACS

    5e5 of anti-FAP CAR-293 cells were stained with 100 μL of 1:25 dilution (4 μL stock solution in 100 μL FACS buffer) of PE-Labeled Human FAP, His Tag (Cat. No. FAP-HP245) and negative control protein respectively. PE signal was used to evaluate the binding activity (QC tested).

    Protocol

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背景介绍

FAP (also known as seprase) is a Type II transmembrane serine protease. Both plasma membrane and soluble forms exhibit post-proline cleaving endopeptidase activity, with a marked preference for Ala/Ser-Gly-Pro-Ser/Asn/Ala consensus sequences. Degrade also gelatin, heat-denatured type I collagen. Also has dipeptidyl peptidase activity, with a preference for Ala-Pro, Ile-Pro, Gly-Pro, Arg-Pro and Pro-Pro. The plasma membrane form, in association with either DPP4, PLAUR or integrins, is involved in the pericellular proteolysis of the extracellular matrix (ECM), and hence promotes cell adhesion, migration and invasion through the ECM. Promotes glioma cell invasion through the brain parenchyma by degrading the proteoglycan brevican. Acts as a tumor suppressor in melanocytic cells through regulation of cell proliferation and survival in a serine protease activity-independent manner.

前沿进展

 
药物研发进展
  • 英文全称:

    Prolyl endopeptidase FAP

  • 中文全称:

    成纤维细胞激活蛋白α

  • 种类:

  • 上市药物数量:

    0 详情

  • 临床药物数量:

    39 详情

  • 最高研发阶段:

    临床二期

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