Human Erythropoietin R / EPO R Protein, Fc Tag (MALS verified)

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ERR-H5253-100ug
¥3675.00
ERR-H5253-1mg (500ug X 2)
¥24255.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    EPOR

  • 表达区间及表达系统(Source)

    Human Erythropoietin R Protein, Fc Tag (ERR-H5253) is expressed from human 293 cells (HEK293). It contains AA Ala 25 - Pro 250 (Accession # P19235-1).

    Predicted N-terminus: Ala 25

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    Erythropoietin R Structure

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 51.3 kDa. The protein migrates as 58-63 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • 内毒素(Endotoxin)

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • 纯度(Purity)

    >90% as determined by SDS-PAGE.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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数据展示

  • 电泳(SDS-PAGE)

    Erythropoietin R SDS-PAGE

    Human Erythropoietin R Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    Erythropoietin R SEC-MALS

    The purity of Human Erythropoietin R Protein, Fc Tag (Cat. No. ERR-H5253) is more than 85% and the molecular weight of this protein is around 100-120 kDa verified by SEC-MALS.

    Report
  • 活性(Bioactivity)-ELISA

     Erythropoietin R ELISA

    Immobilized Human EPO Protein, premium grade (Cat. No. EPO-H4214) at 10 μg/mL (100 μL/well) can bind Human Erythropoietin R Protein, Fc Tag (Cat. No. ERR-H5253) with a linear range of 0.6-10 ng/mL (QC tested).

    Protocol

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背景介绍

This gene encodes the erythropoietin receptor which is a member of the cytokine receptor family. Upon erythropoietin binding, this receptor activates Jak2 tyrosine kinase which activates different intracellular pathways including: Ras/MAP kinase, phosphatidylinositol 3-kinase and STAT transcription factors. The stimulated erythropoietin receptor appears to have a role in erythroid cell survival. Defects in the erythropoietin receptor may produce erythroleukemia and familial erythrocytosis. Dysregulation of this gene may affect the growth of certain tumors. Alternate splicing results in multiple transcript variants.

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