产品详情
分子别名(Synonym)
Erythropoietin R & CD131
表达区间及表达系统(Source)
Human Erythropoietin R & CD131 Protein, Fc Tag&Fc Tag (ERR-H5255) is expressed from human 293 cells (HEK293). It contains AA Ala 25 - Pro 250 & Trp 17 - Trp 443 (Accession # P19235-1 & P32927-1).
Predicted N-terminus: Ala 25 & Trp 17
Request for sequence蛋白结构(Molecular Characterization)

Human Erythropoietin R & CD131 Protein, Fc Tag&Fc Tag is produced by co-expression of Erythropoietin R and CD131, has a calculated MW of 51.3 kDa (Erythropoietin R) & 74.9 kDa (CD131). Subunit Erythropoietin R is fused with a human IgG1 Fc tag at the C-terminus and subunit CD131 is fused with a human IgG1 Fc tag at the C-terminus. The protein migrates as 55-60 kDa and 80-95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a heterodimer.
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method / rFC method.
纯度(Purity)
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系(QMS)
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数据展示
电泳(SDS-PAGE)

Human Erythropoietin R & CD131 Protein, Fc Tag&Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS

The purity of Human Erythropoietin R & CD131 Protein, Fc Tag&Fc Tag (Cat. No. ERR-H5255) is more than 90% and the molecular weight of this protein is around 260-300 kDa verified by SEC-MALS.
Report
活性(Bioactivity)-ELISA

Immobilized Human EPO Protein, premium grade (Cat. No. EPO-H4214) at 10 μg/mL (100 μL/well) can bind Human Erythropoietin R & CD131 Protein, Fc Tag&Fc Tag (Cat. No. ERR-H5255) with a linear range of 0.6-78 ng/mL (QC tested).
Protocol
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