产品详情
分子别名(Synonym)
EPCAM, TACSTD1, TROP1, CD326, DIAR5, EGP2, EGP314, EGP40, ESA, GA733-2, HNPCC8, HNPCC-8, KS1, 4, KSA, M4S1, MIC18, MK1
表达区间及表达系统(Source)
Biotinylated Human EpCAM, Fc,Avitag (EPM-H82F9) is expressed from human 293 cells (HEK293). It contains AA Gln 24 - Lys 265 (Accession # AAH14785.1).
Predicted N-terminus: Gln24
Request for sequence蛋白结构(Molecular Characterization)

This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 56.2 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记(Labeling)
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度(Protein Ratio)
Passed as determined by the HABA assay / binding ELISA.
内毒素(Endotoxin)
Less than 0.1 EU per μg by the LAL method / rFC method.
纯度(Purity)
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
质量管理控制体系(QMS)
推荐产品
数据展示
电泳(SDS-PAGE)

Biotinylated Human EpCAM, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS

The purity of Biotinylated Human EpCAM, Fc,Avitag (Cat. No. EPM-H82F9) is more than 95% and the molecular weight of this protein is around 115-130 kDa verified by SEC-MALS.
Report
活性(Bioactivity)-ELISA

Immobilized Biotinylated Human EpCAM, Fc,Avitag (Cat. No. EPM-H82F9) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-EpCAM Antibody, Human IgG1 with a linear range of 0.2-4 ng/mL (QC tested).
Protocol
用户评价 发表评论

背景介绍
Ep-CAM cross signaling with N-cadherin involves Pi3K, resulting in the abrogation of the cadherin adhesion complexes in epithelial cells was reported. And Epithelial cell adhesion molecule (Ep-CAM) recently received increased attention as a prognostic factor in breast cancer.


















