Japanese encephalitis virus (strain SA-14) Envelope protein E, His Tag (MALS verified)

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货号-规格
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ENE-J52H5-50ug
¥4725.00
ENE-J52H5-500ug
¥28140.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    Envelope protein E/E protein (JEV)

  • 表达区间及表达系统(Source)

    Japanese encephalitis virus (strain SA-14) Envelope protein E, His Tag (ENE-J52H5) is expressed from human 293 cells (HEK293). It contains AA Phe 295 - Ala 700 (Accession # P27395-1).

  • 蛋白结构(Molecular Characterization)

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 45.5 kDa. The protein migrates as 48-53 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

  • 内毒素(Endotoxin)

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • 纯度(Purity)

    >90% as determined by SDS-PAGE.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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数据展示

  • 电泳(SDS-PAGE)

    Envelope protein E/E protein (JEV) SDS-PAGE

    Japanese encephalitis virus (strain SA-14) Envelope protein E, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    Envelope protein E/E protein (JEV) SEC-MALS

    The purity of Japanese encephalitis virus (strain SA-14) Envelope protein E, His Tag (Cat. No. ENE-J52H5) is more than 85% and the molecular weight of this protein is around 40-55 kDa verified by SEC-MALS.

    Report
  • 活性(Bioactivity)-ELISA

     Envelope protein E/E protein (JEV) ELISA

    Immobilized Japanese encephalitis virus (strain SA-14) Envelope protein E, His Tag (Cat. No. ENE-J52H5) at 1 μg/mL (100 μL/well) can bind JEV E-D3 antibody ,Mouse IgG with a linear range of 0.1-2 ng/mL (QC tested).

    Protocol

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背景介绍

Japanese encephalitis B, a mosquito-borne flavivirus, or B encephalitis, is the most important cause of viral encephalitis in Asia in terms of frequency and severity. JEV envelope protein E is an important research target, binding to host cell surface receptors and mediating fusion between the virus and the cell membrane.

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