Human Elongin B&Elongin C&VHL Heterotrimer Protein, Strep II Tag&Strep II Tag&His Tag (MALS&SPR verified)

分子别名（Synonym）

Elongin B & Elongin C & VHL, ELOB & ELOC & VHL Complex, Elongin B & Elongin C & VHL Complex

表达区间及表达系统（Source）

Human Elongin B&Elongin C&VHL Heterotrimer Protein, Strep II Tag&Strep II Tag&His Tag (ELL-H5595) is expressed from Baculovirus-Insect cells. It contains AA Asp 2 - Gln 118 (Elongin B) & Asp 2 - Cys 112 (Elongin C) & Pro 2 - Asp 213 (VHL) (Accession # Q15370-1 (Elongin B) & Q15369-1 (Elongin C) & P40337-2 (VHL)).

Predicted N-terminus: Met (Elongin B) & Met (Elongin C) & Met (VHL)

蛋白结构（Molecular Characterization）

Human Elongin B&Elongin C&VHL Heterotrimer Protein, Strep II Tag&Strep II Tag&His Tag, produced by co-expression of Elongin B and Elongin C and VHL, has a calculated MW of 14.5 kDa (Elongin B) and 13.8 kDa (Elongin C) and 25.8 kDa (VHL). Subunit Elongin B is fused with a Strep II tag at the N-terminus and Elongin C is fused with a Strep II tag at the N-terminus and VHL is fused with a polyhistidine tag at the N-terminus. The protein migrates as 17 kDa (Elongin B) and 14 kDa (Elongin C) and 30 kDa (VHL) under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method / rFC method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Supplied as 0.2 μm filtered solution in 50 mM HEPES, 200 mM NaCl, 20% Glycerol, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

运输（Shipping）

This product is supplied and shipped with dry ice, please inquire the shipping cost.

存储（Storage）

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. The product MUST be stored at -70°C or lower upon receipt;
2. -70°C for 3 months under sterile conditions.

质量管理控制体系（QMS）

1. 质量管理体系（ISO, GMP）
2. 质量优势
3. 质控流程