E. coli Elongation Factor 4 Protein, His Tag (active enzyme)

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EF4-E5143-50ug
¥4500.00
EF4-E5143-500ug
¥26000.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    Elongation Factor 4

  • 表达区间及表达系统(Source)

    E. coli Elongation Factor 4 Protein, His Tag (EF4-E5143) is expressed from E. coli cells. It contains AA Lys 2 - Lys 599 (Accession # C4ZYJ2).

    Predicted N-terminus: Met

    Request for sequence
  • 蛋白结构(Molecular Characterization)

    Elongation Factor 4 Structure

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 68.5 kDa. The protein migrates as 65-80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

  • 纯度(Purity)

    >90% as determined by SDS-PAGE.

  • 制剂(Formulation)

    Supplied as 0.2 μm filtered solution in PBS, 0.5 M NaCl, 1 M Arginine, pH7.4 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • 运输(Shipping)

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • 存储(Storage)

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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  • 电泳(SDS-PAGE)

    Elongation Factor 4 SDS-PAGE

    E. coli Elongation Factor 4 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • 活性(Bioactivity)

    The specific activity of Elongation Factor 4 was determined to be > 1700 pmol/min/mg in a GTPase-Glo assay using GTP solution substrate (QC tested).

    Protocol

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背景介绍

Elongation Factor 4 (EF4), also known as ​​LepA​​, is a highly conserved ​​GTPase translation factor​​ present across bacteria, mitochondria, and chloroplasts. It regulates protein synthesis by interacting with the ribosome during translation elongation. Unlike canonical elongation factors (e.g., EF-G) that promote forward translocation, EF4 catalyzes ​​back-translocation of tRNAs​​ on the ribosome, effectively acting as a molecular ’reverse gear‘ to reposition tRNAs and mRNA. This unique function allows EF4 to maintain translational fidelity under stress conditions (e.g., low pH or high ionic strength) by rescuing stalled ribosomes and correcting errors in protein synthesis.

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