Chikungunya virus (CHIKV) E3 & E2 & E1 Protein, His Tag (MALS verified)

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E3P-V52H3-50ug
¥4725.00
E3P-V52H3-500ug
¥29400.00
合计0件 产品金额¥ 0

产品详情

  • 分子别名(Synonym)

    E3 & E2 & E1

  • 表达区间及表达系统(Source)

    Chikungunya virus (CHIKV) E3 & E2 & E1 Protein, His Tag (E3P-V52H3) is expressed from human 293 cells (HEK293). It contains AA Pro 266 - Arg 325 & Ser 326 - Gln 666 & Tyr 810 - Pro 1200 (Accession # Q1H8W5).

    Predicted N-terminus: Pro 266

  • 蛋白结构(Molecular Characterization)

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 90.6 kDa. The protein migrates as 85-95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

    The protein is designed as a dimer.

  • 纯度(Purity)

    >90% as determined by SDS-PAGE.

  • 制剂(Formulation)

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • 重构方法(Reconstitution)

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • 存储(Storage)

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • 质量管理控制体系(QMS)

    1. 质量管理体系(ISO, GMP)
    2. 质量优势
    3. 质控流程

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  • 电泳(SDS-PAGE)

    E3 & E2 & E1 SDS-PAGE

    Chikungunya virus (CHIKV) E3 & E2 & E1 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    E3 & E2 & E1 SEC-MALS

    The purity of Chikungunya virus (CHIKV) E3 & E2 & E1 Protein, His Tag (Cat. No. E3P-V52H3) is more than 85% and the molecular weight of this protein is around 160-205 kDa verified by SEC-MALS.

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背景介绍

Chikungunya virus (CHIKV) p62 is a structural polyprotein precursor that undergoes furin-mediated cleavage to generate the E3 and E2 glycoproteins. Co-translational association with the fusion protein E1 in the endoplasmic reticulum produces p62–E1 heterodimers that ensure proper folding and spike assembly. Following E3 removal, the mature E2–E1 complex organizes into trimeric spikes on the virion surface, mediating receptor binding and pH-triggered membrane fusion during cell entry. This coordinated processing is essential for CHIKV infectivity and immunogenicity, driving efficient virus assembly.

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